Difference between revisions of "Maintenance"
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* '''<u>Sterile water:</u>''' Always at least one full bottle of autoclaved water | * '''<u>Sterile water:</u>''' Always at least one full bottle of autoclaved water | ||
− | * Always at least one full spray flask of 75% alcohol | + | * '''<u>75% alcohol nspray:</u>''' Always at least one full spray flask of 75% alcohol |
− | * Always one half full bottle of DMEM supplied with extras | + | * '''<u>DMEM ++:</u>'''Always one half full bottle of DMEM supplied with extras |
− | * Yellow waste container no more than 3/4 filled | + | * '''<u>Yellow waste container:</u>''' no more than 3/4 filled |
− | * Check that sticky mat sticks | + | * '''<u>sticky mat :</u>'''Check that sticky mat sticks |
− | * Empty | + | * '''<u>Vacusafe:</u>'''Empty when it is more than half full |
− | * Once a week, refill sterile water in incubator. Need: 1-2 l. | + | * '''<u>Water in incubator:</u>'''Once a week, refill sterile water in incubator. Need: 1-2 l. |
− | * When the diluted PBS bottle is less than half full prepare another | + | * '''<u>Diluted PBS:</u>'''When the diluted PBS bottle is less than half full prepare another |
− | * Check the list of things to buy (on the wall in the fore-room) | + | * '''<u>List:</u>'''Check the list of things to buy (on the wall in the fore-room) |
− | * Check the CO2 level in bottles. Reorder bottle if necessary. | + | * '''<u>CO2:</u>'''Check the CO2 level in bottles. Reorder bottle if necessary. |
− | * Always at least 20 sterile pasteur pipettes in hood, if not: autoclave new | + | * '''<u>Pasteur pipettes:</u>'''Always at least 20 sterile pasteur pipettes in hood, if not: autoclave new |
− | * Every 3 weeks: wash coats | + | * '''<u>Coats:</u>'''Every 3 weeks: wash coats |
Revision as of 13:11, 20 April 2021
Contents
List of things to have all the time in 431
- Sterile water: Always at least one full bottle of autoclaved water
- 75% alcohol nspray: Always at least one full spray flask of 75% alcohol
- DMEM ++:Always one half full bottle of DMEM supplied with extras
- Yellow waste container: no more than 3/4 filled
- sticky mat :Check that sticky mat sticks
- Vacusafe:Empty when it is more than half full
- Water in incubator:Once a week, refill sterile water in incubator. Need: 1-2 l.
- Diluted PBS:When the diluted PBS bottle is less than half full prepare another
- List:Check the list of things to buy (on the wall in the fore-room)
- CO2:Check the CO2 level in bottles. Reorder bottle if necessary.
- Pasteur pipettes:Always at least 20 sterile pasteur pipettes in hood, if not: autoclave new
- Coats:Every 3 weeks: wash coats
List of procedures
Autoclaved water
Must be done 4-5 hours before use of water (for cooling down)
- Get 5l bottles of water from chemistry (check quality resistivity > 1MOhm)
- Fill 1l glass bottle with water
- put cap on, untighten cap
- put autoclave scotch on
- put in autoclave and follow procedure on autoclave
Dulbeccos Modified Eagle Media, DMEM adding extras
- Inside sterile hood with sterile procedures
- Open new bottle of DMEM
- Add 50 ml FBS
- Add 5 ml Penicilin Streptomycin
Vacusafe
- Add chlorine?
- Pour into yellow waste container ?
Refilling water in incubator
- Have 1-2 l sterile water ready and spray top around lid
- Put on gloves and spray gloves
- Open incubator
- withdraw water pan half way
- pour water in to half full
Preparing diluted PBS
- zdf
Autoclaving pasteur pipettes
- stock in cupboard in 431
- put them in a 1l beaker
- ...