Difference between revisions of "20210206 collagen"
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Dagkd@uio.no (talk | contribs) (Created page with "* Took collagen out from freezer at 10:50 === Calculating volumes for aliquoting === * concentration in vials: c= 5 mg/ml * Thin coating = 5 ug/cm^2 * Area of coating: 2-5 cm...") |
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* Area of coating: 2-5 cm^2 per network | * Area of coating: 2-5 cm^2 per network | ||
* Mass of collagen: m= 5*5 = 25ug | * Mass of collagen: m= 5*5 = 25ug | ||
| − | * Volume of undiluted collagen: V = m/c = 0.025 mg/ 5 mg/ml = 0.005 ml = 5 ul, which is 1/1000 of a vial. | + | * Volume of undiluted collagen: V = m/c = 0.025 mg/ 5 mg/ml = 0.005 ml = 5 ul, which is 1/1000 of a vial. It is completely unrealistic to handle such small volumes |
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* If I divide the vial volume into 25 x 200 ul it should give a reasonable amount of aliquots for thin coating | * If I divide the vial volume into 25 x 200 ul it should give a reasonable amount of aliquots for thin coating | ||
Revision as of 12:02, 2 June 2021
- Took collagen out from freezer at 10:50
Calculating volumes for aliquoting
- concentration in vials: c= 5 mg/ml
- Thin coating = 5 ug/cm^2
- Area of coating: 2-5 cm^2 per network
- Mass of collagen: m= 5*5 = 25ug
- Volume of undiluted collagen: V = m/c = 0.025 mg/ 5 mg/ml = 0.005 ml = 5 ul, which is 1/1000 of a vial. It is completely unrealistic to handle such small volumes
- If I divide the vial volume into 25 x 200 ul it should give a reasonable amount of aliquots for thin coating
Diluting and dispensing
- Prepare sterile 17.5M acetic acid stock solution
- Dilute 1:1000 with sterile, distilled H2O
