Difference between revisions of "20210206 collagen"
From mn.fysikk.laglivlab
Dagkd@uio.no (talk | contribs) |
Dagkd@uio.no (talk | contribs) |
||
Line 13: | Line 13: | ||
* Prepare sterile 17.5M acetic acid stock solution | * Prepare sterile 17.5M acetic acid stock solution | ||
* Dilute 1:1000 with sterile, distilled H2O | * Dilute 1:1000 with sterile, distilled H2O | ||
+ | * Concentration of collagen C [ug/ml] = 5 ug/cm^2 / 100 um = 5 ug/ cm^2 / 0.01 cm = 500 ug/ml = 0.5 mg/ml => dilute 1:10 with Acetic acid | ||
+ | * ca 250 ul concentrated collagen + 2250 ul diluted acetic acid |
Revision as of 12:23, 2 June 2021
- Took collagen out from freezer at 10:50
Calculating volumes for aliquoting
- concentration in vials: c= 5 mg/ml
- Thin coating = 5 ug/cm^2
- Area of coating: 2-5 cm^2 per network
- Mass of collagen: m= 5*5 = 25ug
- Volume of undiluted collagen: V = m/c = 0.025 mg/ 5 mg/ml = 0.005 ml = 5 ul, which is 1/1000 of a vial. It is completely unrealistic to handle such small volumes
- If I divide the vial volume into 25 x 200 ul it should give a reasonable amount of aliquots for thin coating
Diluting and dispensing
- Prepare sterile 17.5M acetic acid stock solution
- Dilute 1:1000 with sterile, distilled H2O
- Concentration of collagen C [ug/ml] = 5 ug/cm^2 / 100 um = 5 ug/ cm^2 / 0.01 cm = 500 ug/ml = 0.5 mg/ml => dilute 1:10 with Acetic acid
- ca 250 ul concentrated collagen + 2250 ul diluted acetic acid