Difference between revisions of "20210608 3Dcell labnotes"

Revision as of 16:19, 8 June 2021

2% lowT agarose gel in Falcon tube: 0.18 g in 9 ml (g)water
I made the mistake (once more) to fill the Falcon tube with agarose first and water on top. The water does not penetrate into the agarose because there is air trapped in the powder
Apparently the posts (30 um diameter) do not fill the height of the cell (100 um) Impossible to know how high they are...
Gel fluid is very viscous and I had to keep increasing the pressure. Not sure if this was due to clogging somewhere else or gelling in the cell.
The contact angle varied around 90 degrees
The posts did not alter the fluid pathway at all. This was due to 90 degrees contact angle and very low posts.

PArt of cell with posts not filling the 100 um heigh

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 * I made the mistake (once more) to fill the Falcon tube with agarose first and water on top. The water does not penetrate into the agarose because there is air trapped in the powder
 * Apparently the posts (30 um diameter) do not fill the height of the cell (100 um) Impossible to know how high they are...
+* Gel fluid is very viscous and I had to keep increasing the pressure. Not sure if this was due to clogging somewhere else or gelling in the cell.
+* The contact angle varied around 90 degrees
+* The posts did not alter the fluid pathway at all. This was due to 90 degrees contact angle and very low posts.
 [[File:13330341-2021-06-08-150145.png|left|thumb|PArt of cell with posts not filling the 100 um heigh]]