Difference between revisions of "20210608 3Dcell labnotes"
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| + | * 12:30 PDMS and glass treated at 100% power plasma with O2 let in just before generator turned on | ||
* 2% lowT agarose gel in Falcon tube: 0.18 g in 9 ml (g)water | * 2% lowT agarose gel in Falcon tube: 0.18 g in 9 ml (g)water | ||
* I made the mistake (once more) to fill the Falcon tube with agarose first and water on top. The water does not penetrate into the agarose because there is air trapped in the powder | * I made the mistake (once more) to fill the Falcon tube with agarose first and water on top. The water does not penetrate into the agarose because there is air trapped in the powder | ||
| + | * started filling agarose into circuit at 15:30, 3 hours after plasma treatment. | ||
| + | * Apparently the posts (30 um diameter) do not fill the height of the cell (100 um) Impossible to know how high they are... | ||
| + | * Gel fluid is very viscous and I had to keep increasing the pressure. Not sure if this was due to clogging somewhere else or gelling in the cell. | ||
| + | * The contact angle varied around 90 degrees | ||
| + | * The posts did not alter the fluid pathway at all. This was due to 90 degrees contact angle and very low posts. | ||
| + | |||
| + | [[File:13330341-2021-06-08-150145.png|left|thumb|Part of cell with posts not filling the 100 um heigh]] | ||
| + | |||
| + | [[File:13330341-2021-06-08-161725.png|left|thumb|water-air interface completely unperturbed by "posts"]] | ||
Latest revision as of 12:16, 9 June 2021
- 12:30 PDMS and glass treated at 100% power plasma with O2 let in just before generator turned on
- 2% lowT agarose gel in Falcon tube: 0.18 g in 9 ml (g)water
- I made the mistake (once more) to fill the Falcon tube with agarose first and water on top. The water does not penetrate into the agarose because there is air trapped in the powder
- started filling agarose into circuit at 15:30, 3 hours after plasma treatment.
- Apparently the posts (30 um diameter) do not fill the height of the cell (100 um) Impossible to know how high they are...
- Gel fluid is very viscous and I had to keep increasing the pressure. Not sure if this was due to clogging somewhere else or gelling in the cell.
- The contact angle varied around 90 degrees
- The posts did not alter the fluid pathway at all. This was due to 90 degrees contact angle and very low posts.
