Difference between revisions of "20210610 3Dcell labnotes"

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(Created page with "* 12:30 08 june PDMS and glass treated at 100% power plasma with O2 let in just before generator turned on * 2% lowT agarose gel in Falcon tube: 0.18 g in 9 ml (g)water * 15:0...")
 
 
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* 12:30 08 june PDMS and glass treated at 100% power plasma with O2 let in just before generator turned on
 
* 12:30 08 june PDMS and glass treated at 100% power plasma with O2 let in just before generator turned on
* 2% lowT agarose gel in Falcon tube: 0.18 g in 9 ml (g)water
+
* 1% lowT agarose gel in Falcon tube: 0.1 g in 10 ml (g) DMEM
* 15:00 injected
+
* still difficult to avoid a big lump when I pour the powder in. It should probably be mixed in slowly in a beaker. Cooking skills!
 +
* 15:20 injected agarose in circuit.
 +
* The contact angle is still 90 degrees and the small humps (where the posts should have been) do not stop anything!
 +
[[File:Flowin 2021-06-10-152037-0029.jpg|left|thumb|Agarose flowing in past humps]]

Latest revision as of 15:35, 10 June 2021

  • 12:30 08 june PDMS and glass treated at 100% power plasma with O2 let in just before generator turned on
  • 1% lowT agarose gel in Falcon tube: 0.1 g in 10 ml (g) DMEM
  • still difficult to avoid a big lump when I pour the powder in. It should probably be mixed in slowly in a beaker. Cooking skills!
  • 15:20 injected agarose in circuit.
  • The contact angle is still 90 degrees and the small humps (where the posts should have been) do not stop anything!
Agarose flowing in past humps