Difference between revisions of "20211806 microfluidics"
From mn.fysikk.laglivlab
(Created page with "== Preparing collagen and coating chip with collagen == Collagen preparation: We did a 1:1000 dilution of acetic acid (17.5 M) - we used 10 uL of acetic acid and 10 mL of ste...") (Tag: Visual edit) |
(Tag: Visual edit) |
||
| Line 1: | Line 1: | ||
== Preparing collagen and coating chip with collagen == | == Preparing collagen and coating chip with collagen == | ||
| − | |||
| + | === Collagen preparation: === | ||
We did a 1:1000 dilution of acetic acid (17.5 M) - we used 10 uL of acetic acid and 10 mL of sterile water. | We did a 1:1000 dilution of acetic acid (17.5 M) - we used 10 uL of acetic acid and 10 mL of sterile water. | ||
We did a 2:3 dilution of collagen - we used 200 uL of 5 mg/mL collagen and 300 uL of the diluted acetic acid solution. | We did a 2:3 dilution of collagen - we used 200 uL of 5 mg/mL collagen and 300 uL of the diluted acetic acid solution. | ||
| − | Collagen coating: | + | === Collagen coating: === |
| − | + | We coated Annikens chip (100 um) with collagen. We injected the collagen by using a 5 mL syringe and with a flow of around 50 uL/min. Stopped the flow afterwards and let the collagen settle for one hour. Aspirated the chip by using a 5 mL syringe multiple times. | |
| − | We coated Annikens chip with collagen. | ||
== Casting PDMS == | == Casting PDMS == | ||
Revision as of 14:59, 18 June 2021
Contents
Preparing collagen and coating chip with collagen
Collagen preparation:
We did a 1:1000 dilution of acetic acid (17.5 M) - we used 10 uL of acetic acid and 10 mL of sterile water.
We did a 2:3 dilution of collagen - we used 200 uL of 5 mg/mL collagen and 300 uL of the diluted acetic acid solution.
Collagen coating:
We coated Annikens chip (100 um) with collagen. We injected the collagen by using a 5 mL syringe and with a flow of around 50 uL/min. Stopped the flow afterwards and let the collagen settle for one hour. Aspirated the chip by using a 5 mL syringe multiple times.
Casting PDMS
We casted two new chips - Annikens (100 um) and Thomas' (25 um).
Passaging
We passaged the cells as usual. For detail see here. We kept one flask and changed one flask. We took out 2 ml from the old flask into a 3 ml syringe.
