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| − | == Monthly cleaning and maintenance ==
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| − | * [[Standard items |'''Standard items''']]: Refill and order new if necessary
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| − | * '''Fridge''': Clean and remove items without labels
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| − | * '''[[Vacusafe]]''': Empty when half full
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| − | * '''[[Incubator]]''': Refill sterile water
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| − | == List of things to check regularly in 431 ==
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| − |
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| − | * '''<u>Sterile water:</u>''' Always at least one full bottle of autoclaved water
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| − | * '''<u>75% alcohol spray:</u>''' Always at least one full spray flask of 75% alcohol
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| − | * '''<u>DMEM ++:</u>''' Always one half full bottle of DMEM supplied with extras
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| − | * '''<u>Yellow waste container:</u>''' full, clean then thoroughly before taking them in the lab
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| − | * '''<u>sticky mat:</u>''' Check that sticky mat sticks
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| − | * '''<u>Diluted PBS:</u>''' When the diluted PBS bottle is less than half full prepare another
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| − | * '''<u>CO2:</u>''' Check the CO2 level in bottles. Reorder bottle if necessary.
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| − | * '''<u>Pasteur pipettes:</u>''' Always at least 20 sterile Pasteur pipettes in hood, if not: autoclave new
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| − | * '''<u>Coats:</u>''' Every semester: replace coats
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| − | * '''<u>Water:</u>''' Change water in distilled water small containers
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| − | == Weekly cleaning ==
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| − | Every week the lab has to be cleaned.
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| − | * Move all loose pieces of equipment from lab benches into cupboards and drawers
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| − | * Wipe all surfaces (benches, PC, microscope, ...) with antibac
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| − | * Clean '''LAF bench/hood''' with antibac
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| − | ** Leave only minimum equipment in hood
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| − | ** Remove the base of the bench and clean in the sink
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| − | ** Clean under the base
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| − | ** Clean the inside of the glass sides (windows)
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| − | ** Reassemble
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| − | ** Turn on the UV light
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| − | * Clean inside the centrifuge
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| − | # First clean with distilled water
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| − | # Then clean with ethanol
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| − | # Clean all tube holders with ethanol
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| − | * Mop the floor
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| − | * Spray "what is touched" (handles etc...)
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