Difference between revisions of "20210326 3Dcell labnotes"
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== Pressure control of gel == | == Pressure control of gel == | ||
− | * Set up gel preparation for small amounts of agarose gel (~0.5 ml) | + | * Set up gel preparation for small amounts of [https://www.sigmaaldrich.com/catalog/product/SIGMA/A9045 low gelling temperature agarose gel for cell culture] (~0.5 ml water, ~5 mg agarose) |
* For other gels this can work with even smaller amounts of fluid | * For other gels this can work with even smaller amounts of fluid | ||
* When gel is ready the pressure input (needle) and fluid output (tube) is glued to gas tighten with UV glue | * When gel is ready the pressure input (needle) and fluid output (tube) is glued to gas tighten with UV glue | ||
* Small ID tubing assures little waste of gel | * Small ID tubing assures little waste of gel | ||
− | * Even | + | * Even 3 mbar is enough to flow gel through chip. |
+ | * Changing which channel has higher pressure (by 2-3 mbar) reverses the flow of gelling fluid | ||
+ | * Nice control and it will work when circuit is more hydrophobic! | ||
+ | * Main problems: | ||
+ | ** I had forgotten to sharpen tube (since hole was very small) | ||
+ | ** Difficult to know how long it takes for fluid to reach chip. Fill tube before plugging in chip to have control of when it happens | ||
+ | ** Accidental fluid into chip when I pressed tube in. Go to 0 pressure (and perhaps 3 mbar counterpressure) to avoid accidentaly filling | ||
+ | ** Circuit was not sufficiently hydrophobic | ||
+ | ** There should have been 2-3 posts at end of lamellae to make the difference in capillary pressure greater and avoid breaking through to the wrong side | ||
+ | |||
[[File:Sample holder and chip on microscope.jpg|left|thumb|Sample holder and chip on microscope]] | [[File:Sample holder and chip on microscope.jpg|left|thumb|Sample holder and chip on microscope]] | ||
[[File:PC screen and microscope.jpg|left|thumb|PC screen with image of circuit and microscope]] | [[File:PC screen and microscope.jpg|left|thumb|PC screen with image of circuit and microscope]] |
Latest revision as of 17:33, 26 March 2021
Pressure control of gel
- Set up gel preparation for small amounts of low gelling temperature agarose gel for cell culture (~0.5 ml water, ~5 mg agarose)
- For other gels this can work with even smaller amounts of fluid
- When gel is ready the pressure input (needle) and fluid output (tube) is glued to gas tighten with UV glue
- Small ID tubing assures little waste of gel
- Even 3 mbar is enough to flow gel through chip.
- Changing which channel has higher pressure (by 2-3 mbar) reverses the flow of gelling fluid
- Nice control and it will work when circuit is more hydrophobic!
- Main problems:
- I had forgotten to sharpen tube (since hole was very small)
- Difficult to know how long it takes for fluid to reach chip. Fill tube before plugging in chip to have control of when it happens
- Accidental fluid into chip when I pressed tube in. Go to 0 pressure (and perhaps 3 mbar counterpressure) to avoid accidentaly filling
- Circuit was not sufficiently hydrophobic
- There should have been 2-3 posts at end of lamellae to make the difference in capillary pressure greater and avoid breaking through to the wrong side