Difference between revisions of "Unresolved issues"
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'''Possible solutions to air bubbles''' | '''Possible solutions to air bubbles''' | ||
* The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber. | * The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber. | ||
| + | * Adding soft surfacant to make sure the bubbles detach from the surface. | ||
| + | * [https://darwin-microfluidics.com/products/microfluidic-bubble-trap Bubble Trap for Microfluidics Kit] provided by [https://www.elveflow.com/ Elveflow] which gets rid of the air bubbles before they enter the chip. | ||
| + | *[https://www.elveflow.com/microfluidic-reviews/general-microfluidics/air-bubbles-and-microfluidics/#:~:text=Bubbles%20dissolution%3A%20For%20those%20that,to%20dissolve%20into%20the%20liquid. Air bubbles and microfluidics: Tips and tricks to remove them] | ||
Revision as of 21:04, 31 July 2022
There are several unresolved issues present when it comes to the experiment part of the project.
The following issues are:
Keeping the cells alive inside the chip
Controlling the CO2 levels
Air bubbles
When filling chambers with liquid, the bubbles result in the cells dying prematurely
Possible solutions to air bubbles
- The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
- Adding soft surfacant to make sure the bubbles detach from the surface.
- Bubble Trap for Microfluidics Kit provided by Elveflow which gets rid of the air bubbles before they enter the chip.
- Air bubbles and microfluidics: Tips and tricks to remove them
