Difference between revisions of "24.03.23"

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(Created page with "Present: Nigar, Lasse, Morten Plan for the lab: passaging BV-2 cells (KO and WT), and performing first rotation with KO cells Observations: * higher rpm at peak, possibly i...")
 
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Plan for the lab: passaging BV-2 cells (KO and WT), and performing first rotation with KO cells
 
Plan for the lab: passaging BV-2 cells (KO and WT), and performing first rotation with KO cells
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==== Results (rotations per 10sec):[edit | edit source] ====
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Each row for a clip is a different cell.
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{| class="wikitable"
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!10kHz
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!100kHz
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!200kHz
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!250kHz
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!300kHz
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!350kHz
 +
!400kHz
 +
!500kHz
 +
!1MHz
 +
!5Mhz
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!10MHz
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!15MHz
 +
!20MHz
 +
!30MHz
 +
!40MHz
 +
!50MHz
 +
|-
 +
|0.25
 +
|0
 +
|0
 +
|0
 +
|0
 +
|0
 +
|0
 +
|0
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|0
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|0
 +
|0
 +
|0
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|0
 +
|0
 +
|0
 +
|0
 +
|-
 +
|0.25
 +
|0
 +
|0
 +
|0
 +
|0
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|0
 +
|0
 +
|0
 +
|0
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|0
 +
|0
 +
|0
 +
|0
 +
|0
 +
|0
 +
|0
 +
|-
 +
|0.25
 +
|0
 +
|0
 +
|0
 +
|0
 +
|0
 +
|0
 +
|0
 +
|0
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|0
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|0
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|0
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|0
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|0
 +
|0
 +
|0
 +
|}
  
 
Observations:
 
Observations:

Revision as of 19:21, 27 March 2023

Present: Nigar, Lasse, Morten

Plan for the lab: passaging BV-2 cells (KO and WT), and performing first rotation with KO cells

Results (rotations per 10sec):[edit | edit source]

Each row for a clip is a different cell.

10kHz 100kHz 200kHz 250kHz 300kHz 350kHz 400kHz 500kHz 1MHz 5Mhz 10MHz 15MHz 20MHz 30MHz 40MHz 50MHz
0.25 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0.25 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0.25 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Observations:

  • higher rpm at peak, possibly in the similar frequency range
  • earlier turning point - cells slowed down earlier than WT: were almost stationary at 10MHz
  • larger amount of cell lysis
  • high density of cells : used 2ml og cells instead of 1ml as usual (used 0.5ml for WT on Wednesday, but this is also because the confluency was different)
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