Difference between revisions of "20210419 3Dcell labnotes"
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* The gel is too viscous and the joints between Eppendorf lid and tubes are leaking. | * The gel is too viscous and the joints between Eppendorf lid and tubes are leaking. | ||
* I need a better lid/kork with airtight joints to the tubes. | * I need a better lid/kork with airtight joints to the tubes. | ||
| + | * Made a rubber plug with hole for tube. This plug worked well, no leak it seems | ||
| + | * filling 0.3 mmID teflon tube required more than 100 mbar. When it was filled it flowed at 2 mbar. Hydrophobic capillary | ||
| + | * outlet (channel 2) must have been plugged. This has to be exchanged every time due to hydrogel plugging | ||
| + | * apart from this the water (did not use gel this time) filled lamellae and did not go past pillar. | ||
Latest revision as of 14:42, 19 April 2021
New try of filling.
First observation: UV glue does not stick on Eppendorf
- 0.04g agarose + 1.5 ml water ~ 3% solution put in water bath at 70C
- 0.3 x 0.79 teflon tube into PDMS
- to fit this tube in Eppendorf needed hole of 0.7-0.8 mm. 18G too large, 23G too smalll, 1mm drill best I could find, but it needs filling in to be pressure tight.
- Since glue does not stick on Eppendorf or teflon, use glue, but without UV. This will probably be enough for a few mbar pressure.
- The gel is too viscous and the joints between Eppendorf lid and tubes are leaking.
- I need a better lid/kork with airtight joints to the tubes.
- Made a rubber plug with hole for tube. This plug worked well, no leak it seems
- filling 0.3 mmID teflon tube required more than 100 mbar. When it was filled it flowed at 2 mbar. Hydrophobic capillary
- outlet (channel 2) must have been plugged. This has to be exchanged every time due to hydrogel plugging
- apart from this the water (did not use gel this time) filled lamellae and did not go past pillar.
