Difference between revisions of "20211106 microfluidics"

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- Made 1.6mm holes in the inlets&outlets
 
- Made 1.6mm holes in the inlets&outlets
  
- Plasma cleaned
+
- Plasma cleaned as described in the plasma cleaning test from before.
 +
 
 +
- the 25um high wafer is broken. We will need to create a new 25um mold.
  
 
=== Adding new PDMS to wafer ===
 
=== Adding new PDMS to wafer ===
 
- Used 43.70g PDMS and 3.68 g curing agent.
 
- Used 43.70g PDMS and 3.68 g curing agent.
  
 
+
- We should consider using less PDMS when creating new chips on an already used mold, since they have been too thick.
 
=== Troubles with microfluidic chips ===  
 
=== Troubles with microfluidic chips ===  
 
We had a few problem with punching the chips: starting with Anniken's (?), after some time after plasma it seemed that the chip closed itself (the part facing the air, approx half way in the ). We then tried to re-punch holes out *from the top* which seemed to work.  
 
We had a few problem with punching the chips: starting with Anniken's (?), after some time after plasma it seemed that the chip closed itself (the part facing the air, approx half way in the ). We then tried to re-punch holes out *from the top* which seemed to work.  

Revision as of 16:22, 11 June 2021

Preparing the chip & plasma cleaning

- Cut out Vilde and Dag's chip designs.

- Made 1.6mm holes in the inlets&outlets

- Plasma cleaned as described in the plasma cleaning test from before.

- the 25um high wafer is broken. We will need to create a new 25um mold.

Adding new PDMS to wafer

- Used 43.70g PDMS and 3.68 g curing agent.

- We should consider using less PDMS when creating new chips on an already used mold, since they have been too thick.

Troubles with microfluidic chips

We had a few problem with punching the chips: starting with Anniken's (?), after some time after plasma it seemed that the chip closed itself (the part facing the air, approx half way in the ). We then tried to re-punch holes out *from the top* which seemed to work. To be sure, we cut out another chip (Thomas') but after plasma it turned out to be the same problem. We still managed to get tubes in, watertight.

Why?

- these PDMS chip were coming from the same wafer (broken BTW), which was covered which a very thick layer of PDMS