Difference between revisions of "20211101 Restart"
From mn.fysikk.laglivlab
Dagkd@uio.no (talk | contribs) |
Dagkd@uio.no (talk | contribs) |
||
| Line 11: | Line 11: | ||
=== New microscope glass, cells in microfluidic === | === New microscope glass, cells in microfluidic === | ||
| + | Describe experiment | ||
| − | On Oct 29 2021 Anniken | + | On Oct 29 2021 Anniken's update on the timelapse. The cells did not adhere, but the PDMS was still properly attached to the glass slide. Maybe plasma cleaning the adhesive slides disturbes the cell adhesive properties? Anyway, only plasma cleaning the PDMS didn’t work either. We could try one more time when we have more cells just to be sure or try using fibronectin. |
=== Suggestions for continuation using fibronectin === | === Suggestions for continuation using fibronectin === | ||
Revision as of 12:57, 1 November 2021
Contents
Restart of project
New microscope glass, microfluidic preparation
On Oct 28 2021 Anniken, Adam, Endre and Thomas went to Lab 128 and... PDMS on... Fill in!!
New microscope glass, cells for test
Cells
New microscope glass, cells in microfluidic
Describe experiment
On Oct 29 2021 Anniken's update on the timelapse. The cells did not adhere, but the PDMS was still properly attached to the glass slide. Maybe plasma cleaning the adhesive slides disturbes the cell adhesive properties? Anyway, only plasma cleaning the PDMS didn’t work either. We could try one more time when we have more cells just to be sure or try using fibronectin.
Suggestions for continuation using fibronectin
Anniken has found a protocol for coating with fibronectin and an alternative protocol.
The first recommends 2-10 mg/cm^2, the alternative protocol recommends 1-5 mg/cm^2. We need Tris buffe. Balance and weighing equipment is found in lab 420. ...
