Difference between revisions of "20211101 Restart"
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=== New microscope glass, microfluidic preparation === | === New microscope glass, microfluidic preparation === | ||
| − | On Oct 28 2021 Anniken, Adam, Endre and Thomas went to Lab 128 with the intention of resuming the microfluidic incubator project. PDMS had been prepared in advance. The purpose of meeting was to test adhesive glass in the hopes that they would, electrostatically and/or chemically, attract our cells and anchor them to the slide. Test 1 consisted of plasma treating the PDMS but not the adhesive slide. Test 2 consisted of plasma treating both the PDMS and the adhesive slide. Initially, both tests seemed to generate microfluidic | + | On Oct 28 2021 Anniken, Adam, Endre and Thomas went to Lab 128 with the intention of resuming the microfluidic incubator project. PDMS had been prepared in advance. The purpose of meeting was to test adhesive glass in the hopes that they would, electrostatically and/or chemically, attract our cells and anchor them to the slide. Test 1 consisted of plasma treating the PDMS but not the adhesive slide. Test 2 consisted of plasma treating both the PDMS and the adhesive slide. Initially, both tests seemed to generate microfluidic chip where the PDMS was properly attached to the slides. |
=== New microscope glass, cells for test === | === New microscope glass, cells for test === | ||
| − | + | After having assembled the microfluidic chips, we prepared our cells in order to inject them into the chips. | |
| − | + | The chip from test 1 did not withstand the injection of cells. The PDMS detached from the adhesive slide more or less immediately after being handled. | |
| − | |||
| − | On Oct 29 2021 Anniken's update on the timelapse. The cells did not adhere, but the PDMS was still properly attached to the glass slide. | + | The chip from test 2 did withstand the injection of cells and the PDMS did not detach from the adhesive slide. We started a timelapse to study the cell adhesion. Plasma cleaning the adhesive slide might have disturbed the cell-adhesive properties. |
| + | |||
| + | On Oct 29 2021 Anniken's update on the timelapse. The cells did not adhere, but the PDMS was still properly attached to the glass slide. Plasma cleaning the adhesive slide might have disturbed the cell-adhesive properties. Since we don't know how or with what the adhesive slides are treated, we will move on to trying fibronectin as a coating agent. | ||
=== Suggestions for continuation using fibronectin === | === Suggestions for continuation using fibronectin === | ||
Revision as of 13:46, 2 November 2021
Contents
Restart of project
New microscope glass, microfluidic preparation
On Oct 28 2021 Anniken, Adam, Endre and Thomas went to Lab 128 with the intention of resuming the microfluidic incubator project. PDMS had been prepared in advance. The purpose of meeting was to test adhesive glass in the hopes that they would, electrostatically and/or chemically, attract our cells and anchor them to the slide. Test 1 consisted of plasma treating the PDMS but not the adhesive slide. Test 2 consisted of plasma treating both the PDMS and the adhesive slide. Initially, both tests seemed to generate microfluidic chip where the PDMS was properly attached to the slides.
New microscope glass, cells for test
After having assembled the microfluidic chips, we prepared our cells in order to inject them into the chips.
The chip from test 1 did not withstand the injection of cells. The PDMS detached from the adhesive slide more or less immediately after being handled.
The chip from test 2 did withstand the injection of cells and the PDMS did not detach from the adhesive slide. We started a timelapse to study the cell adhesion. Plasma cleaning the adhesive slide might have disturbed the cell-adhesive properties.
On Oct 29 2021 Anniken's update on the timelapse. The cells did not adhere, but the PDMS was still properly attached to the glass slide. Plasma cleaning the adhesive slide might have disturbed the cell-adhesive properties. Since we don't know how or with what the adhesive slides are treated, we will move on to trying fibronectin as a coating agent.
Suggestions for continuation using fibronectin
Anniken has found a protocol for coating with fibronectin and an alternative protocol.
The first recommends 2-10 mg/cm^2, the alternative protocol recommends 1-5 mg/cm^2. We need Tris buffe. Balance and weighing equipment is found in lab 420.
