Difference between revisions of "20220401 cell adhesion"
From mn.fysikk.laglivlab
(→Plan) |
(→Plan) (Tag: Visual edit) |
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| Line 4: | Line 4: | ||
*1. Bonding | *1. Bonding | ||
| − | *2. Vannfylling | + | *2. Vannfylling (filling in the water) |
*3. Fibronectin | *3. Fibronectin | ||
| − | *4. | + | *4. Cells (w/ bubble trap) |
===Bonding=== | ===Bonding=== | ||
| − | We have ready wafers of our new design | + | We have ready wafers of our new design showed in the figure below. |
| + | [[File:PDMS cast.png|center|thumb]] | ||
| + | '''Procedure''' | ||
| + | # Cut out the PDMS layer, without cutting the petri dish (make sure there is no dust on the scalpel, use tape) | ||
| + | # Once the wafer is removed, make 6 holes with the 1.6 mm punch (make sure the punch is clean before each time you make a hole) | ||
| + | # In the meantime, wash the miscrospoce slide with isopropanol and dry with nitrogen | ||
| + | # Place the wafer inside the plasma cleaner for 1 minute, power: 10090 and flow: 60. | ||
| + | # After it is ready, press PDSM onto the miscroscope slide and make sure it is bonded right away, if not something is wrong | ||
| + | |||
| + | === Filling in the water === | ||
| + | |||
| + | '''Procedure (cell lab)''' | ||
| + | # Prepare the valve (picture) | ||
| + | # Prepare 1 mL syringe + a tube with the smallest inner diameter and needles with the size of 20g or 21g | ||
| + | # Connect the tube to the valve | ||
| + | # Fill the syringe with distilled water, connect to one of the chambers on the wafer and slowly add the water inside, make sure that the water comes out on the other end of the chamber | ||
| + | Once we have added water to the chamber the PDMS layer has disconnected from the microscope slide indicating that there was a mistake when using the plasma cleaner. | ||
Revision as of 15:07, 3 April 2022
Cell Adhesion
Plan
- 1. Bonding
- 2. Vannfylling (filling in the water)
- 3. Fibronectin
- 4. Cells (w/ bubble trap)
Bonding
We have ready wafers of our new design showed in the figure below.
Procedure
- Cut out the PDMS layer, without cutting the petri dish (make sure there is no dust on the scalpel, use tape)
- Once the wafer is removed, make 6 holes with the 1.6 mm punch (make sure the punch is clean before each time you make a hole)
- In the meantime, wash the miscrospoce slide with isopropanol and dry with nitrogen
- Place the wafer inside the plasma cleaner for 1 minute, power: 10090 and flow: 60.
- After it is ready, press PDSM onto the miscroscope slide and make sure it is bonded right away, if not something is wrong
Filling in the water
Procedure (cell lab)
- Prepare the valve (picture)
- Prepare 1 mL syringe + a tube with the smallest inner diameter and needles with the size of 20g or 21g
- Connect the tube to the valve
- Fill the syringe with distilled water, connect to one of the chambers on the wafer and slowly add the water inside, make sure that the water comes out on the other end of the chamber
Once we have added water to the chamber the PDMS layer has disconnected from the microscope slide indicating that there was a mistake when using the plasma cleaner.
