Difference between revisions of "Microfluid incubator"
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== Labratory Notebook == | == Labratory Notebook == | ||
Click here for [[Lab and Planning Notes|Lab and Planning Notes]] | Click here for [[Lab and Planning Notes|Lab and Planning Notes]] | ||
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== Progression Spring Semester 2022 == | == Progression Spring Semester 2022 == | ||
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When trying to integrate the Zimmer & Peacock pH sensor we did not receive the results we were hoping for. The issue of integrating the sensor together with the medium flow is still under planning stage. | When trying to integrate the Zimmer & Peacock pH sensor we did not receive the results we were hoping for. The issue of integrating the sensor together with the medium flow is still under planning stage. | ||
− | == | + | == Microfluid monitoring incubator == |
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+ | === In depth information about the project === | ||
Here's a [https://www.mn.uio.no/fysikk/forskning/prosjekter/laglivlab/dokumenter/laglivlab_spring_2021_projects.pdf presentation] of the concept | Here's a [https://www.mn.uio.no/fysikk/forskning/prosjekter/laglivlab/dokumenter/laglivlab_spring_2021_projects.pdf presentation] of the concept | ||
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+ | Check out this introduction to [https://www.elveflow.com/microfluidic-reviews/microfluidics-for-cell-biology/concepts-and-methodologies/ Cell biology: Microfluidic Concepts and methodologies] | ||
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+ | Scientific paper on following topic | ||
The rage of the 2020s are organs-on-a-chip [1]. These are systems that have to be specifically engineered for each type of organ. Growing and maintaining cell cultures, however is a more generic activity of cell biology. They are kept in expensive incubators and several times a week they have to be looked after and the growth media replaced. When there are too many cells, most of them are killed and some are re-applied in a new flask/petri dish. All this can be done inside a lab on a chip[4]. In addition, the incubator functions of controlling temperature, CO2 and O2 pressure can be reduced to small modules in a standalone cell culture system that is small enough to be moved from lab to lab and between research microscopes at different imaging facilities. Adding electrodes to the system will allow electrochemical or bioimpedance monitoring of the cell coverage, a feature that will be publishable in a scientific journal. | The rage of the 2020s are organs-on-a-chip [1]. These are systems that have to be specifically engineered for each type of organ. Growing and maintaining cell cultures, however is a more generic activity of cell biology. They are kept in expensive incubators and several times a week they have to be looked after and the growth media replaced. When there are too many cells, most of them are killed and some are re-applied in a new flask/petri dish. All this can be done inside a lab on a chip[4]. In addition, the incubator functions of controlling temperature, CO2 and O2 pressure can be reduced to small modules in a standalone cell culture system that is small enough to be moved from lab to lab and between research microscopes at different imaging facilities. Adding electrodes to the system will allow electrochemical or bioimpedance monitoring of the cell coverage, a feature that will be publishable in a scientific journal. |
Revision as of 13:33, 9 July 2022
Contents
Labratory Notebook
Click here for Lab and Planning Notes
Progression Spring Semester 2022
This semester we have worked hard in order to achieve control over CO2 concentration inside the incubator in order to prolong the life of the cells. We have received Zimmer & Peacock ISE OEM, pH sensor and software which we needed to integrate into our experiment in order to measure the pH concentration.
We have used some time to create new wafers with a new design, which we successfully used in our experiments.
When trying to integrate the Zimmer & Peacock pH sensor we did not receive the results we were hoping for. The issue of integrating the sensor together with the medium flow is still under planning stage.
Microfluid monitoring incubator
In depth information about the project
Here's a presentation of the concept
Check out this introduction to Cell biology: Microfluidic Concepts and methodologies
Scientific paper on following topic
The rage of the 2020s are organs-on-a-chip [1]. These are systems that have to be specifically engineered for each type of organ. Growing and maintaining cell cultures, however is a more generic activity of cell biology. They are kept in expensive incubators and several times a week they have to be looked after and the growth media replaced. When there are too many cells, most of them are killed and some are re-applied in a new flask/petri dish. All this can be done inside a lab on a chip[4]. In addition, the incubator functions of controlling temperature, CO2 and O2 pressure can be reduced to small modules in a standalone cell culture system that is small enough to be moved from lab to lab and between research microscopes at different imaging facilities. Adding electrodes to the system will allow electrochemical or bioimpedance monitoring of the cell coverage, a feature that will be publishable in a scientific journal.
The system is perhaps not the most exciting project to start with, but it contains all the basic technology needed for more advanced organ-on-a-chip systems: temperature, gas and fluid control, hydrogel membranes, monitoring of cell state, etc. It will be a platform that LagLivLab students can build on and modify for years.[1] B. Zhang, A. Korolj, B.F.L. Lai, M. Radisic, Advances in organ-on-a-chip engineering, Nat. Rev. Mater. 3 (2018) 257–278. doi:10.1038/s41578-018-0034-7. [4] F. Bunge, S. van den Driesche, M.J. Vellekoop, Microfluidic platform for the long-term on-chip cultivation of mammalian cells for Lab-on-a-Chip applications, Sensors (Switzerland). 17 (2017) 1–15. doi:10.3390/s17071603.