Difference between revisions of "Unresolved issues"
From mn.fysikk.laglivlab
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=== Keeping the cells alive inside the chip === | === Keeping the cells alive inside the chip === | ||
In order to achieve useful results | In order to achieve useful results | ||
| + | |||
| + | '''Possible solutions:''' | ||
=== Controlling the CO2 levels === | === Controlling the CO2 levels === | ||
Environment | Environment | ||
| + | |||
| + | '''Possible solutions:''' | ||
=== '''Air bubbles''' === | === '''Air bubbles''' === | ||
When filling chambers with liquid, the bubbles can result in the cells dying prematurely and/or inhibiting the flow. | When filling chambers with liquid, the bubbles can result in the cells dying prematurely and/or inhibiting the flow. | ||
| − | '''Possible solutions to air bubbles''' | + | '''Possible solutions to air bubbles:''' |
* The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber. | * The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber. | ||
* Adding soft surfacant to make sure the bubbles detach from the surface. | * Adding soft surfacant to make sure the bubbles detach from the surface. | ||
Revision as of 22:20, 31 July 2022
There are several unresolved issues present when it comes to the experiment part of the project.
The following issues are:
Keeping the cells alive inside the chip
In order to achieve useful results
Possible solutions:
Controlling the CO2 levels
Environment
Possible solutions:
Air bubbles
When filling chambers with liquid, the bubbles can result in the cells dying prematurely and/or inhibiting the flow.
Possible solutions to air bubbles:
- The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
- Adding soft surfacant to make sure the bubbles detach from the surface.
- Being highly aware of pressure levels when adding liquids to the chambers. Even the smallest changes in pressure can result in air bubbles.
- Bubble Trap for Microfluidics Kit provided by Elveflow which gets rid of the air bubbles before they enter the chip.
- Air bubbles and microfluidics: Tips and tricks to remove them
- Methods & techniques for perfusion cell culture
