Difference between revisions of "Unresolved issues"
From mn.fysikk.laglivlab
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| + | === Zimmer & Peacock pH sensor === | ||
=== Keeping the cells alive inside the chip === | === Keeping the cells alive inside the chip === | ||
Revision as of 22:02, 9 August 2022
There are several unresolved issues present when it comes to the experiment part of the project.
The following issues are:
Contents
Zimmer & Peacock pH sensor
Keeping the cells alive inside the chip
In order to achieve useful results
Possible solutions:
Controlling the CO2 levels
Environment
Possible solutions:
Air bubbles
When filling chambers with liquid, the bubbles can result in the cells dying prematurely and/or inhibiting the flow.
Possible solutions to air bubbles:
- The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
- Adding soft surfacant to make sure the bubbles detach from the surface.
- Being highly aware of pressure levels when adding liquids to the chambers. Even the smallest changes in pressure can result in air bubbles.
- Bubble Trap for Microfluidics Kit provided by Elveflow which gets rid of the air bubbles before they enter the chip.
- Air bubbles and microfluidics: Tips and tricks to remove them
- Methods & techniques for perfusion cell culture
