Difference between revisions of "24.03.23"

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(Created page with "Present: Nigar, Lasse, Morten Plan for the lab: passaging BV-2 cells (KO and WT), and performing first rotation with KO cells Observations: * higher rpm at peak, possibly i...")
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Revision as of 18:25, 24 March 2023

Present: Nigar, Lasse, Morten

Plan for the lab: passaging BV-2 cells (KO and WT), and performing first rotation with KO cells

Observations:

  • higher rpm at peak, possibly in the similar frequency range
  • earlier turning point - cells slowed down earlier than WT: were almost stationary at 10MHz
  • larger amount of cell lysis
  • high density of cells : used 2ml og cells instead of 1ml as usual (used 0.5ml for WT on Wednesday, but this is also because the confluency was different)