Difference between revisions of "24.03.23"
From mn.fysikk.laglivlab
(Tag: Visual edit) |
(Tag: Visual edit) |
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Revision as of 19:39, 27 March 2023
Present: Nigar, Lasse, Morten
Plan for the lab: passaging BV-2 cells (KO and WT), and performing first rotation with KO cells
Results (rotations per 10sec):[edit | edit source]
Each row for a clip is a different cell.
| 10kHz | 100kHz | 200kHz | 250kHz | 300kHz | 350kHz | 400kHz | 500kHz | 1MHz | 5Mhz | 10MHz | 15MHz | 20MHz | 30MHz | 40MHz | 50MHz |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.25 | 4.25 | 3.25 | 2.75 | 2.75 | 2.75 | 1.75 | 2 | 1 | 0.375 | 0 | 0 | 0 | 0 | 0 | 0 |
| 0.25 | 2.5 | 2.5 | 2.75 | 2.5 | 2 | 2.75 | 1 | 1 | 0.125 | 0 | 0 | 0 | 0 | 0 | 0 |
| 0.25 | 3.25 | 3.25 | 2.25 | 2 | 2.5 | 1.25 | 1.25 | 2.25 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Observations:
- higher rpm at peak, possibly in the similar frequency range
- earlier turning point - cells slowed down earlier than WT: were almost stationary at 10MHz
- larger amount of cell lysis
- high density of cells : used 2ml og cells instead of 1ml as usual (used 0.5ml for WT on Wednesday, but this is also because the confluency was different)
