Difference between revisions of "Cleaning"
From mn.fysikk.laglivlab
(Created page with "== Cleaning and maintenance == === Weekly cleaning === Every week the lab has to be cleaned. * Move all loose pieces of equipment from lab benches into cupboards and drawers *...") |
|||
Line 1: | Line 1: | ||
− | == | + | == List of things to check regularly in 431 == |
+ | |||
+ | * '''<u>Sterile water:</u>''' Always at least one full bottle of autoclaved water | ||
+ | * '''<u>75% alcohol spray:</u>''' Always at least one full spray flask of 75% alcohol | ||
+ | * '''<u>DMEM ++:</u>''' Always one half full bottle of DMEM supplied with extras | ||
+ | * '''<u>Yellow waste container:</u>''' full, clean then thoroughly before taking them in the lab | ||
+ | * '''<u>sticky mat:</u>''' Check that sticky mat sticks | ||
+ | * '''<u>Vacusafe:</u>''' Empty when it is more than half full | ||
+ | * '''<u>Water in incubator:</u>''' Once a week, refill sterile water in incubator. Need: 1-2 l. | ||
+ | * '''<u>Diluted PBS:</u>''' When the diluted PBS bottle is less than half full prepare another | ||
+ | * '''<u>List:</u>''' Check the list of things to buy (on the wall in the fore-room) | ||
+ | * '''<u>CO2:</u>''' Check the CO2 level in bottles. Reorder bottle if necessary. | ||
+ | * '''<u>Pasteur pipettes:</u>''' Always at least 20 sterile Pasteur pipettes in hood, if not: autoclave new | ||
+ | * '''<u>Coats:</u>''' Every semester: replace coats | ||
+ | * '''<u>Water:</u>''' Change water in distilled water small containers | ||
+ | |||
=== Weekly cleaning === | === Weekly cleaning === | ||
Every week the lab has to be cleaned. | Every week the lab has to be cleaned. |
Revision as of 13:03, 17 November 2023
List of things to check regularly in 431
- Sterile water: Always at least one full bottle of autoclaved water
- 75% alcohol spray: Always at least one full spray flask of 75% alcohol
- DMEM ++: Always one half full bottle of DMEM supplied with extras
- Yellow waste container: full, clean then thoroughly before taking them in the lab
- sticky mat: Check that sticky mat sticks
- Vacusafe: Empty when it is more than half full
- Water in incubator: Once a week, refill sterile water in incubator. Need: 1-2 l.
- Diluted PBS: When the diluted PBS bottle is less than half full prepare another
- List: Check the list of things to buy (on the wall in the fore-room)
- CO2: Check the CO2 level in bottles. Reorder bottle if necessary.
- Pasteur pipettes: Always at least 20 sterile Pasteur pipettes in hood, if not: autoclave new
- Coats: Every semester: replace coats
- Water: Change water in distilled water small containers
Weekly cleaning
Every week the lab has to be cleaned.
- Move all loose pieces of equipment from lab benches into cupboards and drawers
- Throw away all unmarked consumables
- Wipe all surfaces (benches, PC, microscope, ...) with antibac
- Clean LAF bench/hood with antibac
- Leave only minimum equipment in hood
- Remove the base of the bench and clean in the sink
- Clean under the base
- Clean the inside of the glass sides (windows)
- Reassemble
- Turn on the UV light
- Clean Incubators (if needed)
- Clean one incubator at the time
- Move all cell flasks etc to the other incubator
- Remove all loose parts and clean all parts and surfaces with antibac
- When needed, decontaminate the incubators (with heat or with peroxide, follow instructions in the manuals).
- Clean fridge
- Remove all unmarked or overdue (check date) containers to trash
- Clean inside the centrifuge
- First clean with distilled water
- Then clean with ethanol
- Clean all tube holders with ethanol
- Mop the floor
- Spray "what is touched" (handles etc...)