Difference between revisions of "Cell culturing"
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== Collagen for 3D or coating surfaces == | == Collagen for 3D or coating surfaces == | ||
− | Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this | + | Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating surfaces, as it can form thin layers for culturing cells. |
− | fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell | ||
− | environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating | ||
− | surfaces, as it can form thin layers for culturing cells. | ||
− | + | [https://ibidi.com/cell-culture-microscopy/107-collagen-type-i-rat-tail.html#/110-concentration_volume-5_mg_ml_4_x_5_ml Type 1 Collagen from Ibidi], '''[https://ibidi.com/img/cms/products/cells_reagents/R_5020X_CollagenI/IN_5020X_CollagenI_05mg.pdf Protocols for use.]''' (bought through Inter Instrument https://interinst.no/. The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA Feb 2021). | |
− | The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA | ||
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Revision as of 14:44, 19 March 2021
Hela cells
Muscle cells
Collagen for 3D or coating surfaces
Type I Collagen is a major structural component of the extracellular matrix (ECM). Therefore, this fibrous protein is often used in three-dimensional (3D) collagen gels that simulate the in vivo cell environment better than the traditional 2D systems. Additionally, Collagen I is ideal for coating surfaces, as it can form thin layers for culturing cells.
Type 1 Collagen from Ibidi, Protocols for use. (bought through Inter Instrument https://interinst.no/. The product number is IB 50202 (4x5ml 5 mg/ml), price 3226,- + MVA Feb 2021).