Difference between revisions of "Maintenance"
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= List of things to have all the time in 431 = | = List of things to have all the time in 431 = | ||
− | * Always at least one full bottle of autoclaved water | + | * '''<u>Sterile water:</u>''' Always at least one full bottle of autoclaved water |
* Always at least one full spray flask of 75% alcohol | * Always at least one full spray flask of 75% alcohol | ||
* Always one half full bottle of DMEM supplied with extras | * Always one half full bottle of DMEM supplied with extras |
Revision as of 13:07, 20 April 2021
Contents
List of things to have all the time in 431
- Sterile water: Always at least one full bottle of autoclaved water
- Always at least one full spray flask of 75% alcohol
- Always one half full bottle of DMEM supplied with extras
- Yellow waste container no more than 3/4 filled
- Check that sticky mat sticks
- Empty Vacusafe when it is more than half full
- Once a week, refill sterile water in incubator. Need: 1-2 l.
- When the diluted PBS bottle is less than half full prepare another
- Check the list of things to buy (on the wall in the fore-room)
- Check the CO2 level in bottles. Reorder bottle if necessary.
- Always at least 20 sterile pasteur pipettes in hood, if not: autoclave new
- Every 3 weeks: wash coats
List of procedures
Autoclaved water
Must be done 4-5 hours before use of water (for cooling down)
- Get 5l bottles of water from chemistry (check quality resistivity > 1MOhm)
- Fill 1l glass bottle with water
- put cap on, untighten cap
- put autoclave scotch on
- put in autoclave and follow procedure on autoclave
Dulbeccos Modified Eagle Media, DMEM adding extras
- Inside sterile hood with sterile procedures
- Open new bottle of DMEM
- Add 50 ml FBS
- Add 5 ml Penicilin Streptomycin
Vacusafe
- Add chlorine?
- Pour into yellow waste container ?
Refilling water in incubator
- Have 1-2 l sterile water ready and spray top around lid
- Put on gloves and spray gloves
- Open incubator
- withdraw water pan half way
- pour water in to half full
Preparing diluted PBS
- zdf
Autoclaving pasteur pipettes
- stock in cupboard in 431
- put them in a 1l beaker
- ...