Difference between revisions of "20210608 3Dcell labnotes"

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(Created page with "* 2% lowT agarose gel in Falcon tube: 0.18 g in 9 ml (g)water * I made the mistake (once more) to fill the Falcon tube with agarose first and water on top. The water does not...")
 
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* 2% lowT agarose gel in Falcon tube: 0.18 g in 9 ml (g)water
 
* 2% lowT agarose gel in Falcon tube: 0.18 g in 9 ml (g)water
 
* I made the mistake (once more) to fill the Falcon tube with agarose first and water on top. The water does not penetrate into the agarose because there is air trapped in the powder
 
* I made the mistake (once more) to fill the Falcon tube with agarose first and water on top. The water does not penetrate into the agarose because there is air trapped in the powder
 +
* Apparently the posts do not fill the height of the cell (100 um) Impossible to know how high they are...
 +
[[File:13330341-2021-06-08-150145.png|left|thumb|PArt of cell with posts not filling the 100 um heigh]]

Revision as of 15:06, 8 June 2021

  • 2% lowT agarose gel in Falcon tube: 0.18 g in 9 ml (g)water
  • I made the mistake (once more) to fill the Falcon tube with agarose first and water on top. The water does not penetrate into the agarose because there is air trapped in the powder
  • Apparently the posts do not fill the height of the cell (100 um) Impossible to know how high they are...
PArt of cell with posts not filling the 100 um heigh