Difference between revisions of "20210629 microfluidics"
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=== Passaging === | === Passaging === | ||
| − | When passaging the cells, we discovered that one of the | + | When passaging the cells, we discovered that one of the flasks was completely covered with mold. We passaged the other flask as normal, splitting it into two new flasks. We also took some of the cells from this flask to inject them into the chip we have covered with collagen. |
Revision as of 15:27, 29 June 2021
PDMS
We made a new batch of PDMS and casted three new chips - Thomas' (25 um), Thomas' (50 um) and Annikens (100 um).
Collagen
We made a new batch of diluted collagen to coat Thomas' chip from last time (25.06.2021). We used 10 uL of 17.5 M acetic acid and 10 mL of sterilized water to make a acetic acid dilution. Then we took 300 uL of this dilution and mixed it with 200 uL of collagen. We began injecting the collagen into the tubes using the syringe pump but decided there were too many bubbles in the tubes. We kept what was left of the collagen dilution. Then we mixed a new batch of diluted collagen and put everything into a new syringe to try again. Total amount of collagen was 0.5 mL. We managed to fill the chip with collagen without bubbles.
Passaging
When passaging the cells, we discovered that one of the flasks was completely covered with mold. We passaged the other flask as normal, splitting it into two new flasks. We also took some of the cells from this flask to inject them into the chip we have covered with collagen.
