Difference between revisions of "20211101 Restart"
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=== Suggestions for continuation using fibronectin === | === Suggestions for continuation using fibronectin === | ||
− | [https://wiki.uio.no/mn/fysikk/laglivlab/images/2/22/Suggested_Procedure_for_Coating_Cell_Cultureware.pdf | + | Anniken has found a |
+ | [https://wiki.uio.no/mn/fysikk/laglivlab/images/2/22/Suggested_Procedure_for_Coating_Cell_Cultureware.pdf protocol for coating with fibronectin] and an [https://wiki.uio.no/mn/fysikk/laglivlab/images/d/d5/Alternativ_protokoll.pdf alternative protocol]. | ||
+ | |||
+ | The first recommends 2-10 mg/cm^2, the alternative protocol recommends 1-5 mg/cm^2. We need Tris buffe. Balance and weighing equipment is found in lab 420. | ||
+ | ... |
Revision as of 12:44, 1 November 2021
Contents
Restart of project
New microscope glass, microfluidic preparation
On Oct 28 2021 Anniken, Adam, Endre and Thomas went to Lab 128 and... PDMS on... Fill in!!
New microscope glass, cells for test
Cells
New microscope glass, cells in microfluidic
On Oct 29 2021 Anniken registered that the cells did not adhere... Fill in
Suggestions for continuation using fibronectin
Anniken has found a protocol for coating with fibronectin and an alternative protocol.
The first recommends 2-10 mg/cm^2, the alternative protocol recommends 1-5 mg/cm^2. We need Tris buffe. Balance and weighing equipment is found in lab 420. ...