Difference between revisions of "20211101 Restart"
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=== New microscope glass, microfluidic preparation === | === New microscope glass, microfluidic preparation === | ||
| − | On Oct 28 2021 Anniken, Adam, Endre and Thomas went to Lab 128 with the intention of resuming the microfluidic incubator project. ... PDMS | + | On Oct 28 2021 Anniken, Adam, Endre and Thomas went to Lab 128 with the intention of resuming the microfluidic incubator project. PDMS had been prepared in advance. The purpose of meeting was to test adhesive glass in the hopes that they would, electrostatically and/or chemically, attract our cells and anchor them to the slide. Test 1 consisted of plasma treating the PDMS but not the adhesive slide. Test 2 consisted of plasma treating both the PDMS and the adhesive slide. Initially, both tests seemed to generate microfluidic chips where the PDMS was properly attached to the slides. |
=== New microscope glass, cells for test === | === New microscope glass, cells for test === | ||
Revision as of 13:31, 2 November 2021
Contents
Restart of project
New microscope glass, microfluidic preparation
On Oct 28 2021 Anniken, Adam, Endre and Thomas went to Lab 128 with the intention of resuming the microfluidic incubator project. PDMS had been prepared in advance. The purpose of meeting was to test adhesive glass in the hopes that they would, electrostatically and/or chemically, attract our cells and anchor them to the slide. Test 1 consisted of plasma treating the PDMS but not the adhesive slide. Test 2 consisted of plasma treating both the PDMS and the adhesive slide. Initially, both tests seemed to generate microfluidic chips where the PDMS was properly attached to the slides.
New microscope glass, cells for test
Cells
New microscope glass, cells in microfluidic
Describe experiment
On Oct 29 2021 Anniken's update on the timelapse. The cells did not adhere, but the PDMS was still properly attached to the glass slide. Maybe plasma cleaning the adhesive slides disturbes the cell adhesive properties? Anyway, only plasma cleaning the PDMS didn’t work either. We could try one more time when we have more cells just to be sure or try using fibronectin.
Suggestions for continuation using fibronectin
Anniken has found a protocol for coating with fibronectin and an alternative protocol.
The first recommends 2-10 mg/cm^2, the alternative protocol recommends 1-5 mg/cm^2. We need Tris buffe. Balance and weighing equipment is found in lab 420.
