Cleaning
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Revision as of 13:18, 17 November 2023 by Siljabla@uio.no (talk | contribs)
Monthly cleaning and maintenance
- Standard items: Refill and order new if necessary
- Fridge: Clean and remove items without labels
- Vacusafe: Empty when half full
- Incubator: Refill sterile water
List of things to check regularly in 431
- Sterile water: Always at least one full bottle of autoclaved water
- 75% alcohol spray: Always at least one full spray flask of 75% alcohol
- DMEM ++: Always one half full bottle of DMEM supplied with extras
- Yellow waste container: full, clean then thoroughly before taking them in the lab
- sticky mat: Check that sticky mat sticks
- Diluted PBS: When the diluted PBS bottle is less than half full prepare another
- CO2: Check the CO2 level in bottles. Reorder bottle if necessary.
- Pasteur pipettes: Always at least 20 sterile Pasteur pipettes in hood, if not: autoclave new
- Coats: Every semester: replace coats
- Water: Change water in distilled water small containers
Weekly cleaning
Every week the lab has to be cleaned.
- Move all loose pieces of equipment from lab benches into cupboards and drawers
- Throw away all unmarked consumables
- Wipe all surfaces (benches, PC, microscope, ...) with antibac
- Clean LAF bench/hood with antibac
- Leave only minimum equipment in hood
- Remove the base of the bench and clean in the sink
- Clean under the base
- Clean the inside of the glass sides (windows)
- Reassemble
- Turn on the UV light
- Clean Incubators (if needed)
- Clean one incubator at the time
- Move all cell flasks etc to the other incubator
- Remove all loose parts and clean all parts and surfaces with antibac
- When needed, decontaminate the incubators (with heat or with peroxide, follow instructions in the manuals).
- Clean fridge
- Remove all unmarked or overdue (check date) containers to trash
- Clean inside the centrifuge
- First clean with distilled water
- Then clean with ethanol
- Clean all tube holders with ethanol
- Mop the floor
- Spray "what is touched" (handles etc...)