20210319 3Dcell labnotes

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Casting PDMS 10:1

  • poured about 40 ml into disposable beaker on the balance: 32.2g
  • poured curing agent by hand until weight was 35.4 g
  • mixed. lots of bubbles in mixture
  • put beaker in vacuum chamber
  • cycled vacuum / air several times to make and break bubbles
  • continued until almost all bubbles gone
  • poured carefully over mask and put into oven

Preparing for the next steps

  • 4 medium and 2 big circuits made were not destroyed by breaking the wafer
  • Find cover slips and clean. They all fit on 18x24mm cs found in drawer by plasma machine
  • Cut loose and peel off master
  • punch holes for 1/16" tubes
  • clean with tape
  • plasma treat PDMS and CS
  • bond
  • connect tubes
  • fill circuits with distilled water