20210319 3Dcell labnotes

Casting PDMS 10:1

• poured about 40 ml into disposable beaker on the balance: 32.2g
• poured curing agent by hand until weight was 35.4 g
• mixed. lots of bubbles in mixture
• put beaker in vacuum chamber
• cycled vacuum / air several times to make and break bubbles
• continued until almost all bubbles gone
• poured carefully over mask and put into oven

Preparing for the next steps

Making the mufluid circuits

In room 118

• 4 medium and 2 big circuits made were not destroyed by breaking the wafer
• Find cover slips and clean. They all fit on 18x24mm cs found in drawer by plasma machine
• Cut loose and peel off master
• punch holes for 1/16" tubes (which punch? test!)
• clean with tape
• plasma treat PDMS and CS
• bond
• connect tubes

Filling with water and gel

In room 431. If we wait long time between bonding the circuit and filling it the PDMS will become hydrophobic. The main idea of the procedure is that the PDMS should be slightly hydrophobic, but not too much to get the gel into the side chambers, but not to pass the column.

• fill circuits with distilled water

=== Aliquoting