20210419 3Dcell labnotes
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Revision as of 13:34, 19 April 2021 by Dagkd@uio.no (talk | contribs) (Created page with "New try of filling. First observation: UV glue does not stick on Eppendorf * 0.04g agarose + 1.5 ml water ~ 3% solution put in water bath at 70C * 0.3 x 0.79 teflon tube in...")
New try of filling.
First observation: UV glue does not stick on Eppendorf
- 0.04g agarose + 1.5 ml water ~ 3% solution put in water bath at 70C
- 0.3 x 0.79 teflon tube into PDMS
- to fit this tube in Eppendorf needed hole of 0.7-0.8 mm. 18G too large, 23G too smalll, 1mm drill best I could find, but it needs filling in to be pressure tight.
- Since glue does not stick on Eppendorf or teflon, use glue, but without UV. This will probably be enough for a few mbar pressure.
- The gel is too viscous and the joints between Eppendorf lid and tubes are leaking.
- I need a better lid/kork with airtight joints to the tubes.
