24.03.23

Present: Nigar, Lasse, Morten

Plan for the lab: passaging BV-2 cells (KO and WT), and performing first rotation with KO cells

Results (rotations per 10sec):[edit | edit source]

Each row for a clip is a different cell.

10kHz	100kHz	200kHz	250kHz	300kHz	350kHz	400kHz	500kHz	1MHz	5Mhz	10MHz	15MHz	20MHz	30MHz	40MHz	50MHz
0.25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
0.25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
0.25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

Observations:

• higher rpm at peak, possibly in the similar frequency range
• earlier turning point - cells slowed down earlier than WT: were almost stationary at 10MHz
• larger amount of cell lysis
• high density of cells : used 2ml og cells instead of 1ml as usual (used 0.5ml for WT on Wednesday, but this is also because the confluency was different)