05.05.23
From mn.fysikk.laglivlab
Revision as of 10:28, 7 May 2023 by Lassetot@uio.no (talk | contribs)
Present:
- Nigar
- Lasse
- Morten
- Felix
Results (rotations per 10sec):
Each row for a clip is a different cell.
10kHz | 100kHz | 200kHz | 300kHz | 400kHz | 500kHz | 1MHz | 5Mhz | 15MHz | 20MHz | 30MHz | 40MHz | 50MHz |
---|---|---|---|---|---|---|---|---|---|---|---|---|
0.5 | 3.125 | 3 | 2.125 | 1.875 | 1.5 | 0.5 | 1.125 | -0.25 | -0.375 | -0.75 | -1 | -0.75 |
0.375 | 1.25 | 1.125 | 2.215 | 1.75 | 1.125 | 1 | 0.875 | -0.375 | -0.25 | -1.125 | -1 | -0.75 |
0.375 | 2.125 | 2.625 | 1.625 | 1.875 | 1.625 | 1.125 | 0.5 | -0.25 | -0.375 | -0.625 | -1 | -0.875 |
Observations:
- We did a rotation on KO cells. For some reason, we identified a lot of cell lysate, so somethng mght have gone wrong during passaging, or with the EDTA (maybe it is too old). We acquired data nevertheless, and uploaded on the shared Google Drive.
- We were a bit inefficient in starting the experiment after preparing the sample, which may also have affected the data.
- Some cells in the 200-500 khz range were *a lot* faster than all their neighbours, so they were ignored as outliers.
- Data plotted below. In right figure we get a similar average spectra from all our KO data, no apparent significant change from 14.04.23.
![](/mn/fysikk/laglivlab/images/1/1d/BV2_KO_WT_%281%29.png)
The average KO rotation spectra is not significantly changed from the results seen from 14.04.23.