Cleaning

List of things to check regularly in 431

• Sterile water: Always at least one full bottle of autoclaved water
• 75% alcohol spray: Always at least one full spray flask of 75% alcohol
• DMEM ++: Always one half full bottle of DMEM supplied with extras
• Yellow waste container: full, clean then thoroughly before taking them in the lab
• sticky mat: Check that sticky mat sticks
• Vacusafe: Empty when it is more than half full
• Water in incubator: Once a week, refill sterile water in incubator. Need: 1-2 l.
• Diluted PBS: When the diluted PBS bottle is less than half full prepare another
• List: Check the list of things to buy (on the wall in the fore-room)
• CO2: Check the CO2 level in bottles. Reorder bottle if necessary.
• Pasteur pipettes: Always at least 20 sterile Pasteur pipettes in hood, if not: autoclave new
• Coats: Every semester: replace coats
• Water: Change water in distilled water small containers

Weekly cleaning

Every week the lab has to be cleaned.

• Move all loose pieces of equipment from lab benches into cupboards and drawers
• Throw away all unmarked consumables
• Wipe all surfaces (benches, PC, microscope, ...) with antibac
• Clean LAF bench/hood with antibac
  • Leave only minimum equipment in hood
  • Remove the base of the bench and clean in the sink
  • Clean under the base
  • Clean the inside of the glass sides (windows)
  • Reassemble
  • Turn on the UV light
• Clean Incubators (if needed)
  • Clean one incubator at the time
  • Move all cell flasks etc to the other incubator
  • Remove all loose parts and clean all parts and surfaces with antibac
  • When needed, decontaminate the incubators (with heat or with peroxide, follow instructions in the manuals).
• Clean fridge
  • Remove all unmarked or overdue (check date) containers to trash
• Clean inside the centrifuge

1. First clean with distilled water
2. Then clean with ethanol
3. Clean all tube holders with ethanol

• Mop the floor
• Spray "what is touched" (handles etc...)