20210325 3Dcell labnotes
From mn.fysikk.laglivlab
Revision as of 20:08, 24 March 2021 by Dagkd@uio.no (talk | contribs) (Created page with "== Plan for the day == === Equipment === * 0.5 ml Eppendorf tubes * tube rack for holding Eppendorfs upright * pipette * distilled water (or growth medium) * low gelling temp...")
Plan for the day
Equipment
- 0.5 ml Eppendorf tubes
- tube rack for holding Eppendorfs upright
- pipette
- distilled water (or growth medium)
- low gelling temperature agarose
- small beaker
- heater
- precision balance
- microscope at 37C
Gel procedure
- start microscope heater to 37C
- put circuit and pipettes inside microscope box to heat to 37 C
- heat water bath in small beaker to 60C on hot plate
- for 0.5 ml 1 wt % agarose gel
- 0.5 g = 500 mg water = 500 ul
- weigh 5 mg agarose
- put eppendorf in water bath and mix with pipette while heating
Gel into microfluidic procedure
- Start PC and camera on microscope, choose magnification and test imaging of circuit
- connect 2 ml syringe to output of gel channel
- prepare connection of ul micropipette to input of channel
- withdraw tube/syringe tip from circuit inlet
- Fill ul pipette with agarose gel solution
- reconnect ul pipette to gel channel input
- On microscope with camera recording
- inject gel
- adjust counter pressure with outlet syringe to get lamella filled
- when filled, aspire from inlet syringe and press with outlet syringe to get an open channel between lamellae
