List of things to check regularly in 431

Sterile water: Always at least one full bottle of autoclaved water
75% alcohol spray: Always at least one full spray flask of 75% alcohol
DMEM ++: Always one half full bottle of DMEM supplied with extras
Yellow waste container: no more than 3/4 filled
sticky mat : Check that sticky mat sticks
Vacusafe: Empty when it is more than half full
Water in incubator: Once a week, refill sterile water in incubator. Need: 1-2 l.
Diluted PBS: When the diluted PBS bottle is less than half full prepare another
List: Check the list of things to buy (on the wall in the fore-room)
CO2: Check the CO2 level in bottles. Reorder bottle if necessary.
Pasteur pipettes: Always at least 20 sterile pasteur pipettes in hood, if not: autoclave new
Coats: Every 3 weeks: wash coats

List of things to check regularly in 420

So far the activities here do not require constant resupplies

Must be done 4-5 hours before use of water (for cooling down)

Get 5L bottles of water from chemistry (check quality resistivity > 1 Ohms) or use the stock in 420
Fill 1l glass bottle with water
put cap on, untighten it slightly
put autoclave scotch the lid and the bottle. One piece of scotch should cover both
put in autoclave and follow procedure on autoclave

Put on gloves and spray gloves
Have 1-2L sterile water (autoclaved type II, not type I) ready and spray the outside
Open incubator
Withdraw water pan half way
Pour water in to half full (around 1.5L total)
Once a month, get the tray out, empty it completely and clean it with ethanol before filling it back. While the tray is out, swipe remains of water at the bottom of the incubator and clean this part with ethanol.