20211806 microfluidics
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Revision as of 14:55, 18 June 2021 by Annibjor@uio.no (talk | contribs) (Created page with "== Preparing collagen and coating chip with collagen == Collagen preparation: We did a 1:1000 dilution of acetic acid (17.5 M) - we used 10 uL of acetic acid and 10 mL of ste...")
Preparing collagen and coating chip with collagen
Collagen preparation:
We did a 1:1000 dilution of acetic acid (17.5 M) - we used 10 uL of acetic acid and 10 mL of sterile water.
We did a 2:3 dilution of collagen - we used 200 uL of 5 mg/mL collagen and 300 uL of the diluted acetic acid solution.
Collagen coating:
We coated Annikens chip with collagen.
Casting PDMS
We casted two new chips - Annikens (100 um) and Thomas' (25 um).
Passaging
We passaged the cells as usual. For detail see here. We kept one flask and changed one flask. We took out 2 ml from the old flask into a 3 ml syringe.
