20211806 microfluidics

Preparing collagen and coating chip with collagen

Collagen preparation:

We did a 1:1000 dilution of acetic acid (17.5 M) - we used 10 uL of acetic acid and 10 mL of sterile water.

We did a 2:3 dilution of collagen - we used 200 uL of 5 mg/mL collagen and 300 uL of the diluted acetic acid solution.

Collagen coating:

We coated Annikens chip (100 um) with collagen. We injected the collagen by using a 5 mL syringe and with a flow of around 50 uL/min. Stopped the flow afterwards and let the collagen settle for one hour. Aspirated the chip by using a 5 mL syringe multiple times.

Casting PDMS

We casted two new chips - Annikens (100 um) and Thomas' (25 um).

Passaging

We passaged the cells as usual. For detail see here. We kept one flask and changed one flask. We took out 2 ml from the old flask into a 3 ml syringe.