Difference between revisions of "Unresolved issues"
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* [https://darwin-microfluidics.com/products/microfluidic-bubble-trap Bubble Trap for Microfluidics Kit] provided by [https://www.elveflow.com/ Elveflow] which gets rid of the air bubbles before they enter the chip. | * [https://darwin-microfluidics.com/products/microfluidic-bubble-trap Bubble Trap for Microfluidics Kit] provided by [https://www.elveflow.com/ Elveflow] which gets rid of the air bubbles before they enter the chip. | ||
*[https://www.elveflow.com/microfluidic-reviews/general-microfluidics/air-bubbles-and-microfluidics/#:~:text=Bubbles%20dissolution%3A%20For%20those%20that,to%20dissolve%20into%20the%20liquid. Air bubbles and microfluidics: Tips and tricks to remove them] | *[https://www.elveflow.com/microfluidic-reviews/general-microfluidics/air-bubbles-and-microfluidics/#:~:text=Bubbles%20dissolution%3A%20For%20those%20that,to%20dissolve%20into%20the%20liquid. Air bubbles and microfluidics: Tips and tricks to remove them] | ||
| + | *[https://www.elveflow.com/microfluidic-reviews/microfluidics-for-cell-biology/methods-and-techniques-perfusion-cell-culture/ Methods & techniques for perfusion cell culture] | ||
Revision as of 22:14, 31 July 2022
There are several unresolved issues present when it comes to the experiment part of the project.
The following issues are:
Keeping the cells alive inside the chip
Controlling the CO2 levels
Air bubbles
When filling chambers with liquid, the bubbles can result in the cells dying prematurely and/or inhibiting the flow.
Possible solutions to air bubbles
- The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
- Adding soft surfacant to make sure the bubbles detach from the surface.
- Being highly aware of pressure levels when adding liquids to the chambers. Even the smallest changes in pressure can result in air bubbles.
- Bubble Trap for Microfluidics Kit provided by Elveflow which gets rid of the air bubbles before they enter the chip.
- Air bubbles and microfluidics: Tips and tricks to remove them
- Methods & techniques for perfusion cell culture
