Difference between revisions of "Unresolved issues"
From mn.fysikk.laglivlab
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=== Keeping the cells alive inside the chip === | === Keeping the cells alive inside the chip === | ||
| + | In order to achieve useful results | ||
=== Controlling the CO2 levels === | === Controlling the CO2 levels === | ||
| + | Environment | ||
=== '''Air bubbles''' === | === '''Air bubbles''' === | ||
Revision as of 22:19, 31 July 2022
There are several unresolved issues present when it comes to the experiment part of the project.
The following issues are:
Keeping the cells alive inside the chip
In order to achieve useful results
Controlling the CO2 levels
Environment
Air bubbles
When filling chambers with liquid, the bubbles can result in the cells dying prematurely and/or inhibiting the flow.
Possible solutions to air bubbles
- The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
- Adding soft surfacant to make sure the bubbles detach from the surface.
- Being highly aware of pressure levels when adding liquids to the chambers. Even the smallest changes in pressure can result in air bubbles.
- Bubble Trap for Microfluidics Kit provided by Elveflow which gets rid of the air bubbles before they enter the chip.
- Air bubbles and microfluidics: Tips and tricks to remove them
- Methods & techniques for perfusion cell culture
