Unresolved issues
From mn.fysikk.laglivlab
Revision as of 21:19, 31 July 2022 by Krisheye@uio.no (talk | contribs)
There are several unresolved issues present when it comes to the experiment part of the project.
The following issues are:
Keeping the cells alive inside the chip
Controlling the CO2 levels
Air bubbles
When filling chambers with liquid, the bubbles can result in the cells dying prematurely and/or inhibiting the flow.
Possible solutions to air bubbles
- The chip design itself. By creating a design in which there are no sharp angles can reduce the chance of air bubbles adhering to the surface of the chamber.
- Adding soft surfacant to make sure the bubbles detach from the surface.
- Being highly aware of pressure levels when adding liquids to the chambers. Even the smallest changes in pressure can result in air bubbles.
- Bubble Trap for Microfluidics Kit provided by Elveflow which gets rid of the air bubbles before they enter the chip.
- Air bubbles and microfluidics: Tips and tricks to remove them
