Difference between revisions of "Main Page"
From mn.ibv.metagenomics
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| − | + | = CEES metagenomics wiki = | |
| + | This wiki is meant as a resource for people performing metagenomics work in CEES. The website contains protocols for DNA extraction, PCR, library preps, sequencing etc., in addition to relevant literature. | ||
| − | + | == Material == | |
| − | == | + | The starting material currently includes cod gut samples, human stool samples and (...?). |
| − | * [//www.mediawiki.org/wiki/Manual:Configuration_settings Configuration settings list] | + | |
| − | * [//www.mediawiki.org/wiki/Manual:FAQ MediaWiki FAQ] | + | == Protocols == |
| − | * [https://lists.wikimedia.org/mailman/listinfo/mediawiki-announce MediaWiki release mailing list] | + | |
| − | * [//www.mediawiki.org/wiki/Localisation#Translation_resources Localise MediaWiki for your language] | + | === DNA extraction === |
| + | |||
| + | For the above mentioned materials, the main procedure for DNA extraction is a modified version of the DNeasy Blood & Tissue Kit, including an initial bead beating step. | ||
| + | |||
| + | [http://www.qiagen.com/no/resources/download.aspx?id=6b09dfb8-6319-464d-996c-79e8c7045a50&lang=en DNeasy 96 Blood & Tissue Kit handbook] | ||
| + | |||
| + | Paper describing the modified protocol: "[http://www.google.no/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0CCQQFjAA&url=http%3A%2F%2Fwww.researchgate.net%2Fprofile%2FChristopher_Allender%2Fpublication%2F8167483_High-throughput_extraction_of_arthropod_vector_and_pathogen_DNA_using_bead_milling%2Flinks%2F09e4150871fd43c853000000.pdf&ei=5NS8VPvjCMn2OoCygYgF&usg=AFQjCNFwgGWnboyaAEUiNdOUSxuccghlsQ&bvm=bv.83829542,d.ZWU High-throughput extraction of arthropod vector and pathogen DNA using bead milling]" (Allender et al. 2004) | ||
| + | |||
| + | |||
| + | |||
| + | == Resources == | ||
| + | |||
| + | *[[//www.mediawiki.org/wiki/Manual:Configuration_settings|Configuration settings list]] | ||
| + | *[[//www.mediawiki.org/wiki/Manual:FAQ|MediaWiki FAQ]] | ||
| + | *[https://lists.wikimedia.org/mailman/listinfo/mediawiki-announce MediaWiki release mailing list] | ||
| + | *[[//www.mediawiki.org/wiki/Localisation#Translation_resources|Localise MediaWiki for your language]] | ||
Revision as of 11:59, 19 January 2015
CEES metagenomics wiki
This wiki is meant as a resource for people performing metagenomics work in CEES. The website contains protocols for DNA extraction, PCR, library preps, sequencing etc., in addition to relevant literature.
Material
The starting material currently includes cod gut samples, human stool samples and (...?).
Protocols
DNA extraction
For the above mentioned materials, the main procedure for DNA extraction is a modified version of the DNeasy Blood & Tissue Kit, including an initial bead beating step.
DNeasy 96 Blood & Tissue Kit handbook
Paper describing the modified protocol: "High-throughput extraction of arthropod vector and pathogen DNA using bead milling" (Allender et al. 2004)
