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CEES metagenomics wiki

This wiki is meant as a resource for people performing metagenomics work in CEES. The website contains protocols for DNA extraction, PCR, library preps, sequencing etc., in addition to relevant literature.

Tips for adding a page (from the bioinf-wiki of Lex).


The starting material currently includes cod gut samples, human stool samples and (...?).


DNA extraction and 16S rDNA amplification are performed according to the protocols of the Earth MIcrobiome Project (EMP). The protocols can be found here.

DNA extraction

MoBio Powersoil HTP 96-well Soil DNA isolation kit, slightly modified by EMP:

16S rDNA PCR amplification

EMP protocol:

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