Neurotransporter Atlas: VGLUT3

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A: Punctuate dark-brown labeling representing VGLUT3-positive nerve endings in the pyramidal layer of the hippocampus. Scale bar, 50 µm.
B: VGLUT3 stained neuronal cell bodies (arrows) and processes (arrowheads) in the striatum. Scale bar, 50 µm.

Vesicular glutamate transporters (VGLUT1-3) carry glutamate into synaptic vesicles. The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain, visualized by immunohistochemistry using antibodies against VGLUT3.

Access image repository

The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.

Re-use of data from this repository is allowed provided that reference is given to the following publication: Stensrud MJ, Chaudhry FA, Leergaard TB, Bjaalie JG, Gundersen V. Vesicular glutamate transporter-3 in the rodent brain: vesicular colocalization with vesicular γ-aminobutyric acid transporter. J Comp Neurol (2013) 521:3042–56. doi:10.1002/cne.23331

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Experimental procedures in brief

The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).

Bregma levels were determined using a standard stereotaxic atlas of the rat brain (Paxinos and Watson, The rat brain in stereotaxic coordinates, Elsevier 2008) brain. For further details, see Stensrud et al. (Neuroscience 162:1055-71, 2009)

Contributing laboratories

The Neuro- and Gliotransmitter Group, Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Experimental work, immunohistochemistry. People: Mats Stensrud, Grazyna Babinska, Vidar Gundersen

Neural Systems Laboratory (http://www.nesys.uio.no), Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Image acquisition, atlas repository. People: Dmitri Darine, Gergely Csucs, Trygve B. Leergaard, Jan G. Bjaalie

Funded by:

The neuroinformatics components of this resource have been funded by the Human Brain Project through the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement no. 604102 (HBP), and The Research Council of Norway (INCF National Node, project 218842).

Contact

j.g.bjaalie@medisin.uio.no