TTA / TetOff Atlas III (Nop)

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The tTA atlas III (Nop) is an interactive resource providing access to brain-wide histological images documenting the activity of the Nop-tTA promoter in bigenic mice in which the Nop-tTA promoter regulates the expression of the LacZ reporter gene, encoding the histologically detectible marker β-galactosidase. Detailed knowledge about where in the brain gene expression is regulated is essential for the use and interpretation of such models.

Access image repository

The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.Re-use of data from this repository is allowed provided that reference is given to the following publication: Yetman MJ, Lillehaug S, Bjaalie JG, Leergaard TB, Jankowsky JL. Transgene expression in the Nop-TTA driver line is not inherently restricted to the entorhinal cortex. Brain Struct Funct. 2015. DOI 10.1007/s00429-015-1040-9

Animal # Genotype Section orientation Image repository Download images
2849 Nop-tTA/tetO-lacZ-nls-GFP Coronal Filmstrip viewer Tiffs
1952 Nop-tTA/tetO-lacZ-nls-GFP Coronal Filmstrip viewer Tiffs
2877 Nop-tTA/tetO-lacZ-nls-GFP Horizontal Filmstrip viewer Tiffs
1950 tetO-lacZ-nls-GFP (control) Coronal Filmstrip viewer Tiffs

Experimental procedures in brief

The atlas is based on data from adult 4-6 month old bigenic Nop-tTA/tetO-lacZ-nls-GFP mice and adult 4-6 month old single transgenic tetO-lacZ-nls-GFP mice (controls). Sagittal sections from right hemisphere were cryosectioned at 40 µm. β-galactosidase was labeled blue using X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) as a substrate. Weak counterstaining with Neutral Nuclear Fast Red facilitated detection of boundaries and landmarks without compromising the observation of X-gal labeling. Digital images were created using a slidescanner (AxioScan Z1, Carl Zeiss Microimaging, Jena, Germany).

Contributing laboratories

Departments of Neuroscience1, Neurology, and Neurosurgery2, Huffington Center on Aging1,2, Baylor College of Medicine, Houston, TX, USA: Animal breeding and immunohistochemistry. People: Michael J. Yetman1, Joanna L. Jankowsky

Neural Systems Laboratory (, Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Atlas development, neuroanatomical analyses. People: Sveinung Lillehaug, Dmitri Darine, Gergely Csucs, Trygve B. Leergaard, Jan G. Bjaalie

Funded by

  • NIH (DP2 OD001734 and T32 AG000183)
  • The Research Council of Norway (214842; and NORBRAIN, The Norwegian Large Scale Infrastructure for Brain Research)
  • The neuroinformatics components of this resource were funded by the Human Brain Project through the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement no. 604102 (HBP)
  • Uninett Sigma