Difference between revisions of "Neurotransporter Atlas: VGLUT3"
From projects.navigator3
| Line 1: | Line 1: | ||
| − | <span style="font-family:georgia,serif"><span style="color: rgb(0, 0, 0)">The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain. The present | + | <span style="font-family:georgia,serif;"><span style="color: rgb(0, 0, 0)">The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain. The present viewing tool allows the user to navigate within and across sagittal section images. The atlas is based on data presented in: </span><br/></span> |
| + | <span style="font-family:georgia,serif;">Stensrud MJ, Chaudhry FA, Leergaard TB, Bjaalie JG, Gundersen V. Vesicular glutamate transporter-3 in the rodent brain: vesicular colocalization with vesicular γ-aminobutyric acid transporter. J Comp Neurol (2013) 521:3042–56. doi:10.1002/cne.23331</span> | ||
| + | <span style="font-family:georgia,serif;"><br/></span> | ||
| − | == <span style="font- | + | == <span style="font-family:georgia,serif;"><span style="font-size:medium"><span style="color: rgb(0, 0, 0); font-weight: bold">VGLUT3 labeling as visible in high-magnification images</span></span></span> == |
{| style="width: 400px" border="0" cellpadding="0" cellspacing="0" | {| style="width: 400px" border="0" cellpadding="0" cellspacing="0" | ||
|- | |- | ||
| − | | [[File:FigureA VGLUT3.png|thumb|left|400px|A: Punctuate dark-brown labeling representing VGLUT3-positive nerve endings in the pyramidal layer of the hippocampus. Scale bar, 50 µm.]]<br/> | + | | <span style="font-family:georgia,serif;">[[File:FigureA VGLUT3.png|thumb|left|400px|A: Punctuate dark-brown labeling representing VGLUT3-positive nerve endings in the pyramidal layer of the hippocampus. Scale bar, 50 µm.]]<br/></span> |
| − | | [[File:FigureB VGLUT3.png|thumb|left|437px|B: VGLUT3 stained neuronal cell bodies (arrows) and processes (arrowheads) in the striatum. Scale bar, 50 µm.]]<br/> | + | | <span style="font-family:georgia,serif;">[[File:FigureB VGLUT3.png|thumb|left|437px|B: VGLUT3 stained neuronal cell bodies (arrows) and processes (arrowheads) in the striatum. Scale bar, 50 µm.]]<br/></span> |
|} | |} | ||
| + | <span style="font-family:georgia,serif;"><br/></span> | ||
| + | <div style="clear:both"></div> | ||
| + | == <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="color: rgb(0, 0, 0); font-weight: bold">Experimental procedures</span></span></span> == | ||
| − | < | + | <span style="font-family:georgia,serif;">The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).</span> |
| − | == <span style="font- | + | |
| + | == <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="color: rgb(0, 0, 0); font-weight: bold">Atlas coordinate values</span></span></span> == | ||
| − | <span style="font-family:georgia,serif"> | + | <span style="font-family:georgia,serif;">Positional coordinates representing distance from Bregma are indicated for each image in the repository. Values were estimated by matching by evaluation of multiple anatomical landmarks in comparison to a standard rat brain atlas (Paxinos and Watson, The Rat Brain in Stereotaxic Coordinates, Elsevier Academic Press, 2007).</span> |
| − | == <span style="font- | + | == <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="color: rgb(0, 0, 0); font-weight: bold">Data sharing</span></span></span> == |
| − | <span style="font-family:georgia,serif"> | + | <span style="font-family:georgia,serif;">Re-use of data from this repository is permitted provided reference is made to the following publication: Stensrud et al. J Comp Neurol, 521:3042-56, 2013. doi: 10.1002/cne.23331.</span> |
| − | == <span style="font- | + | == <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="color: rgb(0, 0, 0); font-weight: bold"></span></span></span> == |
| − | <span style="font-family:georgia,serif"> | + | == <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="color: rgb(0, 0, 0); font-weight: bold">Access repository and Virtual Microscope</span></span></span> == |
| − | + | <span style="font-family:georgia,serif;">Go to Navigator to access and explore the high resolution section images.</span> | |
| − | <span style="font-family: | + | == <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="font-weight: bold"></span></span></span> == |
| − | == <span style="font-family:georgia,serif"><span style="font-size:smaller"><span style="font-weight: bold">Contributing laboratories</span></span></span> == | + | == <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="font-weight: bold">Contributing laboratories</span></span></span> == |
| − | <span style="font-family:georgia,serif">'''The Neuro- and Gliotransmitter Group''' - Vidar Gundersen</span> | + | <span style="font-family:georgia,serif;">'''The Neuro- and Gliotransmitter Group''' - Vidar Gundersen</span> |
| − | <span style="font-family:georgia,serif">Institute of Basic Medical Sciences - Anatomy<br/>University of Oslo<br/>P.O. Box 1105 Blindern<br/>N - 0317 Oslo<br/>Norway<br/>[http://www.med.uio.no/imb/english/research/groups/synaptic-neurochemistry/gliotransmitters/index.html http://www.med.uio.no/imb/english/research/groups/synaptic-neurochemistry/gliotransmitters/index.html]<br/></span> | + | <span style="font-family:georgia,serif;">Institute of Basic Medical Sciences - Anatomy<br/>University of Oslo<br/>P.O. Box 1105 Blindern<br/>N - 0317 Oslo<br/>Norway<br/>[http://www.med.uio.no/imb/english/research/groups/synaptic-neurochemistry/gliotransmitters/index.html http://www.med.uio.no/imb/english/research/groups/synaptic-neurochemistry/gliotransmitters/index.html]<br/></span> |
| + | <span style="font-family:georgia,serif;"><br/></span> | ||
| + | <span style="font-family:georgia,serif;">'''Neural Systems and Graphics Computing Laboratory''' - Trygve B. Leergaard, Jan G. Bjaalie<br/>Institute of Basic Medical Sciences - Anatomy<br/>University of Oslo<br/>P.O. Box 1105 Blindern<br/>N - 0317 Oslo<br/>Norway<br/>[http://www.nesys.uio.no/ http://www.nesys.uio.no]<br/>[http://www.rbwb.org/ http://www.rbwb.org]<br/><br/></span> | ||
| − | <span style="font-family:georgia,serif"> | + | == <span style="font-family:georgia,serif;"><br/><span style="font-size:smaller"><span style="font-weight: bold">Credits</span></span></span> == |
| − | == | + | === <span style="font-family:georgia,serif;"><span style="font-size:smaller">Immunohistochemistry</span></span> === |
| − | + | <span style="font-family:georgia,serif;"><span style="font-size:small">Grazyna Babinska<br/>Mats Julius Stensrud<br/>Vidar Gundersen</span></span> | |
| − | <span style="font-family:georgia,serif"><span style="font-size: | + | === <span style="font-family:georgia,serif;"><span style="font-size:smaller">NT-atlas web-application development team</span></span> === |
| − | + | <span style="font-family:georgia,serif;"><span style="font-size:small">Dmitri Darine<br/>Gergely Csucs<br/>Trygve B. Leergaard<br/>Jan G. Bjaalie</span></span> | |
| − | <span style="font-family:georgia,serif"><span style="font-size: | + | == <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="font-weight: bold"></span></span></span> == |
| − | == <span style="font-family:georgia,serif"><span style="font-size:smaller"><span style="font-weight: bold">Requirements</span></span></span> == | + | == <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="font-weight: bold">Requirements</span></span></span> == |
Revision as of 12:41, 17 February 2015
The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain. The present viewing tool allows the user to navigate within and across sagittal section images. The atlas is based on data presented in:
Stensrud MJ, Chaudhry FA, Leergaard TB, Bjaalie JG, Gundersen V. Vesicular glutamate transporter-3 in the rodent brain: vesicular colocalization with vesicular γ-aminobutyric acid transporter. J Comp Neurol (2013) 521:3042–56. doi:10.1002/cne.23331
Contents
VGLUT3 labeling as visible in high-magnification images
| |
|
Experimental procedures
The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).
Atlas coordinate values
Positional coordinates representing distance from Bregma are indicated for each image in the repository. Values were estimated by matching by evaluation of multiple anatomical landmarks in comparison to a standard rat brain atlas (Paxinos and Watson, The Rat Brain in Stereotaxic Coordinates, Elsevier Academic Press, 2007).
Data sharing
Re-use of data from this repository is permitted provided reference is made to the following publication: Stensrud et al. J Comp Neurol, 521:3042-56, 2013. doi: 10.1002/cne.23331.
Access repository and Virtual Microscope
Go to Navigator to access and explore the high resolution section images.
Contributing laboratories
The Neuro- and Gliotransmitter Group - Vidar Gundersen
Institute of Basic Medical Sciences - Anatomy
University of Oslo
P.O. Box 1105 Blindern
N - 0317 Oslo
Norway
http://www.med.uio.no/imb/english/research/groups/synaptic-neurochemistry/gliotransmitters/index.html
Neural Systems and Graphics Computing Laboratory - Trygve B. Leergaard, Jan G. Bjaalie
Institute of Basic Medical Sciences - Anatomy
University of Oslo
P.O. Box 1105 Blindern
N - 0317 Oslo
Norway
http://www.nesys.uio.no
http://www.rbwb.org
Credits
Immunohistochemistry
Grazyna Babinska
Mats Julius Stensrud
Vidar Gundersen
NT-atlas web-application development team
Dmitri Darine
Gergely Csucs
Trygve B. Leergaard
Jan G. Bjaalie
