Difference between revisions of "Neurotransporter Atlas: VGLUT3"
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Vesicular glutamate transporters (VGLUT1-3) carry glutamate into synaptic vesicles. The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain, visualized by immunohistochemistry using antibodies against VGLUT3. | Vesicular glutamate transporters (VGLUT1-3) carry glutamate into synaptic vesicles. The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain, visualized by immunohistochemistry using antibodies against VGLUT3. | ||
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| + | |[[File:R601.png|75px|center]] | ||
| + | |S1/M1 forelimb, BDA (R601) | ||
| + | |[http://cmbn-navigator.uio.no/navigator/filmstrip_viewer.html?publicOnly=true&publicOnly=true&entityType=block&entityId=1941&selectIndex=60&metadataKeyName=Bregma%20Level Filmstrip viewer] | ||
| + | |[[media:Metadata_R601.pdf|Details (pdf)]] | ||
| + | |[http://cmbn-navigator.uio.no/navigator/feeder/all_originals/?id=1941 R601 (tiff)] | ||
== <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="color: rgb(0, 0, 0); font-weight: bold">Access image repository</span></span></span> == | == <span style="font-family:georgia,serif;"><span style="font-size:smaller"><span style="color: rgb(0, 0, 0); font-weight: bold">Access image repository</span></span></span> == | ||
Revision as of 13:42, 21 June 2016
Contents
About
Vesicular glutamate transporters (VGLUT1-3) carry glutamate into synaptic vesicles. The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain, visualized by immunohistochemistry using antibodies against VGLUT3.
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|S1/M1 forelimb, BDA (R601) |Filmstrip viewer |Details (pdf) |R601 (tiff)
Access image repository
The virtual microscopy viewer allows interactive zooming and panning. Original images are available for download via separate link.
Re-use of data from this repository is allowed provided that reference is given to the following publication: Stensrud MJ, Chaudhry FA, Leergaard TB, Bjaalie JG, Gundersen V. Vesicular glutamate transporter-3 in the rodent brain: vesicular colocalization with vesicular γ-aminobutyric acid transporter. J Comp Neurol (2013) 521:3042–56. doi:10.1002/cne.23331
| Image repository | Download |
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| Filmstrip viewer | Tiffs |
Experimental procedures in brief
The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).
Bregma levels were determined using a standard stereotaxic atlas of the rat brain (Paxinos and Watson, The rat brain in stereotaxic coordinates, Elsevier 2008) brain. For further details, see Stensrud et al. (Neuroscience 162:1055-71, 2009)
Contributing laboratories
The Neuro- and Gliotransmitter Group, Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Experimental work, immunohistochemistry. People: Mats Stensrud, Grazyna Babinska, Vidar Gundersen
Neural Systems Laboratory (http://www.nesys.uio.no), Centre for Molecular Biology and Neuroscience & Institute of Basic Medical Sciences, Department of Anatomy, University of Oslo, P.O. Box 1105 Blindern, N - 0317 Oslo, Norway: Image acquisition, atlas repository. People: Dmitri Darine, Gergely Csucs, Trygve B. Leergaard, Jan G. Bjaalie
Funded by:
The neuroinformatics components of this resource have been funded by the Human Brain Project through the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement no. 604102 (HBP)
Contact
j.g.bjaalie@medisin.uio.no
