Neurotransporter Atlas: VGLUT3

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The VGLUT3-atlas provides online access to high resolution images revealing the distribution of vesicular glutamate transporter 3 (VGLUT3) in the normal rodent brain. The present Virtual Microscope viewing tool allows the user to navigate within and across sagittal section images. The atlas is based on data published by Stensrud et al. 2013 (J Comp Neurol, 521:3042-56).

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VGLUT3 labeling as visible in high-magnification images

Experimental procedures

The images represent sections of one male Wistar rat (8 weeks). The sections were cut on a freeze microtome and labeled with antibodies against VGLUT3 (0,17µg/ml, polyclonal rabbit antibody, catalog number 135 203, Synaptic Systems) according to a standard protocol for light microscopic immunoperoxidase cytochemistry (Gundersen et al. J Neurosci, 18:6059-6070, 1998).

Atlas coordinate values

Positional coordinates representing distance from Bregma are indicated for each image in the repository. Values were estimated by matching by evaluation of multiple anatomical landmarks in comparison to a standard rat brain atlas (Paxinos and Watson, The Rat Brain in Stereotaxic Coordinates, Elsevier Academic Press, 2007).

Data sharing:

Re-use of data from this repository is permitted provided reference is made to the following publication: Stensrud et al. J Comp Neurol, 521:3042-56, 2013. doi: 10.1002/cne.23331.

Access repository and Virtual Microscope

<span style="font-size:larger;"Contributing laboratories</span>

The Neuro- and Gliotransmitter Group - Vidar Gundersen

Centre for Molecular Biology and Neuroscience &
Institute of Basic Medical Sciences - Anatomy
University of Oslo
P.O. Box 1105 Blindern
N - 0317 Oslo
Norway
http://www.med.uio.no/imb/english/research/groups/synaptic-neurochemistry/gliotransmitters/index.html
http://www.cmbn.no

Neural Systems and Graphics Computing Laboratory - Trygve B. Leergaard, Jan G. Bjaalie
Centre for Molecular Biology and Neuroscience &
Institute of Basic Medical Sciences - Anatomy
University of Oslo
P.O. Box 1105 Blindern
N - 0317 Oslo
Norway
http://www.nesys.uio.no
http://www.rbwb.org
http://www.cmbn.no


Credits

Immunohistochemistry

Grazyna Babinska
Mats Julius Stensrud
Vidar Gundersen

NT-atlas web-application development team:

Dmitri Darine
Gergely Csucs
Trygve B. Leergaard
Jan G. Bjaalie


Requirements

Web browser requirement: