10.03.23

We did our first trial rotation with BV-2 WT cells. We used 0.5ml from 10ml cell concentrate.

Observations:

• during the recording, some cells were moving towards the electrode, which is an issue for 50 MHz, so we took 2 recordings.
• observed rotation below 10KHz, but it was very slow so we did not record that
• the peak was in the 100Khz range. The peak did not feel as sharp as with HeLa, more of a plateu. This will be investigated further after plotting the rotational spectra.
• Spectra appears to be distinct from our HeLa spectra, but we will gather more data on this cell line before determining a control spectra

We are also trying to figure out the optimal cell concentration for cell culturing.

Results (rotations per 10sec):

Each row for a clip is a different cell.

10kHz	100kHz	200kHz	300kHz	500kHz	1MHz	5Mhz	10MHz	20MHz	30MHz	40MHz	50MHz
0.25	2	2.75	3	3.25	1.5	1.25	0.625	-0.25	-1	-0.75	-1
0.125	1.5	2.75	3.5	2.5	2.25	1.25	0.25	-0.25	-0.75	-1.25	-1
0.125	1.5	3.75	3.25	2.5	2.75	0.75	0.5	-0.25	-0.5	-1	-1.25

First BV-2 WT rotation spectra (data in table above), performed by LLL electrorotation group